Filters: Tags: qPCR (X)
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This dataset provides cycle threshold (Ct) values achieved during qPCR for 6 sex-linked genes, and two autosomal genes, for individuals from 60 species and across 13 orders of birds.
We conducted a study to test the factors related to detectability of two invasive aquatic plants (Egeria densa and Myriophyllym spicatum) using environmental DNA (eDNA), over extended periods of time, and specifically examined how plant growth stage and abundance relates to eDNA detection in semi-natural and natural conditions. This dataset is from sampling performed in summer of 2018 in lakes with varying species abundances, and a subset of lakes were re-sampled to test temporal variability in detection.
Categories: Data;
Tags: Aquatic Biology,
Downloadable Data,
Ecology,
Egeria densa,
Myriophyllym spicatum,
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in situ field samples that were tested to the verify assay.
Detection of environmental DNA (eDNA) has become a commonly used surveillance method for threatened or invasive vertebrates in both aquatic and terrestrial environments. However, use of eDNA methodologies for the detection of aquatic invertebrates (e.g., crayfish and insects) has been limited. Environmental DNA protocols can be especially useful for endangered invertebrates such as the Hine’s emerald dragonfly (Somatochlora hineana) where conservation efforts have been greatly hindered by the training, time, overall costs, and environmental impacts associated with conducting surveys in the calcareous fens occupied by this species. An essential step in developing such a protocol is to evaluate the dynamics of eDNA...
Categories: Data;
Tags: Aquatic Biology,
Door County, Wisconsin,
HED,
Hine's Emerald Dragonfly,
USGS Science Data Catalog (SDC),
Concerns exist that the degradation of water quality in Salado Creek near Salado, Texas could adversely affect recreational uses and harm salamander populations that live in and near springs that discharge into the stream. Salado is part of the rapidly growing Killeen-Temple-Fort Hood area; land-use changes in the Salado Creek watershed could adversely affect water quality in Salado Creek. Streamflow in Salado Creek is generally derived from the Salado Springs complex during low-flow conditions (dry weather) and from the springs and surface runoff during high-flow conditions (wet weather). A better understanding of the types of waste (human, animal, or both) that may be entering the stream and springs is needed...
A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes Proliferative Kidney Disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. We present data that was used to determine assay sensitivity, specificity and to define the limits of detection and quantification.
Categories: Data;
Types: Citation;
Tags: East Gallatin River,
Montana,
Sypes Canyon Creek,
Tetracapsuloides bryosalmonae,
Yellowstone River,
This dataset contains environmental DNA (eDNA) concentrations and spawning adult abundances for sockeye salmon (Oncorhynchus nerka) in Hansen Creek, a small tributary in the Wood River watershed, southeast Alaska, USA. We took temporally- and spatially-replicated eDNA samples during the spawning period (mid-July through August), along with fish counts (live, naturally dead, and killed), dissolved oxygen (DO), discharge, and temperature data at each time of sampling. Samples were collected at each site before adults entered the stream, and then continued to be collected daily or every other day for the next three weeks.
Categories: Data;
Types: Downloadable,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
Shapefile;
Tags: Alaska,
Aleknagik Lake,
CO3,
DNeasy,
Hansen Creek,
Spectaclecase (Margaritifera monodonta) is a federally endangered freshwater mussel species that has experienced a 55% reduction in range (USFWS 2014) and is currently concentrated in three rivers in the Midwest of the United States (Gasconade, Meramec Rivers, MO, and St. Croix River, WI). Its preference for living under large rocks and boulders has limited detection of new populations by traditional survey methods. Environmental DNA technology has been used to detect invasive and rare species, but its use for detection of rare, benthic-dwelling species in large flowing systems has been limited. Here, we propose using environmental DNA to identify presumable sites for discovery of M. monodonta. We designed a M....
Categories: Data;
Tags: Illinois,
Margaritifera monodonta,
Minnesota,
St Croix River,
USGS Science Data Catalog (SDC),
In 2018 and 2019, a multidisciplinary team from the U.S. Geological Survey (USGS), National Park Service, The Commonwealth Scientific and Industrial Research Organization, Montana State University’s Department of Microbiology and Immunology, and the Teton Conservation District, initiated a study to document the presence or absence of Naegleria fowleri, Giardia, Escherichia coli, and Cryptosporidium at several hot springs in Grand Teton National Park and John D. Rockefeller Jr. Memorial Parkway and Bridger-Teton National Forest, Yellowstone National Park, and Lake Mead National Recreation Area. This data release includes qPCR data from this investigation.
Categories: Data;
Tags: Aquatic Biology,
Arizona,
Arizona Hot Spring,
Astoria Hot Springs,
Blue Point Spring,
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