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We conducted a study to test the factors related to detectability of two invasive aquatic plants (Egeria densa and Myriophyllym spicatum) using environmental DNA (eDNA), over extended periods of time, and specifically examined how plant growth stage and abundance relates to eDNA detection in semi-natural and natural conditions. This dataset is from sampling performed in summer of 2018 in lakes with varying species abundances, and a subset of lakes were re-sampled to test temporal variability in detection.
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in situ field samples that were tested to the verify assay.
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Concerns exist that the degradation of water quality in Salado Creek near Salado, Texas could adversely affect recreational uses and harm salamander populations that live in and near springs that discharge into the stream. Salado is part of the rapidly growing Killeen-Temple-Fort Hood area; land-use changes in the Salado Creek watershed could adversely affect water quality in Salado Creek. Streamflow in Salado Creek is generally derived from the Salado Springs complex during low-flow conditions (dry weather) and from the springs and surface runoff during high-flow conditions (wet weather). A better understanding of the types of waste (human, animal, or both) that may be entering the stream and springs is needed...
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A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes Proliferative Kidney Disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. We present data that was used to determine assay sensitivity, specificity and to define the limits of detection and quantification.
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This dataset contains environmental DNA (eDNA) concentrations and spawning adult abundances for sockeye salmon (Oncorhynchus nerka) in Hansen Creek, a small tributary in the Wood River watershed, southeast Alaska, USA. We took temporally- and spatially-replicated eDNA samples during the spawning period (mid-July through August), along with fish counts (live, naturally dead, and killed), dissolved oxygen (DO), discharge, and temperature data at each time of sampling. Samples were collected at each site before adults entered the stream, and then continued to be collected daily or every other day for the next three weeks.
Spectaclecase (Margaritifera monodonta) is a federally endangered freshwater mussel species that has experienced a 55% reduction in range (USFWS 2014) and is currently concentrated in three rivers in the Midwest of the United States (Gasconade, Meramec Rivers, MO, and St. Croix River, WI). Its preference for living under large rocks and boulders has limited detection of new populations by traditional survey methods. Environmental DNA technology has been used to detect invasive and rare species, but its use for detection of rare, benthic-dwelling species in large flowing systems has been limited. Here, we propose using environmental DNA to identify presumable sites for discovery of M. monodonta. We designed a M....


    map background search result map search result map Quantitative PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples Concentrations of environmental DNA (eDNA) during sockeye salmon spawning in 2016, Hansen Creek, Alaska, USA Nutrients, Escherichia coli, and microbial source tracking markers in surface-water and known-source samples collected in Salado Creek and its watershed near Salado, Texas, 2018 Detection of invasive aquatic plants Myriophyllum spicatum and Egeria densa in lakes using eDNA, field and mesocosm data Transformation methods for glochidia of the Spectaclecase mussel Cumberlandia monodonta: Data Mucket eDNA detection in Wallen’s Bend, Clinch river, Tennessee, September 2019 Mucket eDNA detection in Wallen’s Bend, Clinch river, Tennessee, September 2019 Concentrations of environmental DNA (eDNA) during sockeye salmon spawning in 2016, Hansen Creek, Alaska, USA Nutrients, Escherichia coli, and microbial source tracking markers in surface-water and known-source samples collected in Salado Creek and its watershed near Salado, Texas, 2018 Detection of invasive aquatic plants Myriophyllum spicatum and Egeria densa in lakes using eDNA, field and mesocosm data Transformation methods for glochidia of the Spectaclecase mussel Cumberlandia monodonta: Data Quantitative PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples