Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea.
Dates
Publication Date
2023-11-13
Start Date
2019-10-16
End Date
2021-07-19
Citation
Klymus, K.E., Ruiz-Ramos, D.V., Thompson, N.L., Voshage, M.C. and Richter, C.A., 2023, Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea: U.S. Geological Survey data release, https://doi.org/10.5066/P9IVYWPG.
Summary
Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
Summary
Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
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Purpose
Environmental DNA data for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea were collected from laboratory experiments to estimate DNA shedding rates under controlled environmental conditions. These rates will improve our understanding of mussel eDNA detection in the field, ultimately improving our ability to use eDNA for monitoring mussel populations.
