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This dataset contains data tables of laboratory quality-control data associated with environmental samples analyzed for microbiological constituents at the Ohio Water Microbiology Laboratory of the U.S. Geological Survey (USGS). The environmental samples were collected across the United States by USGS National Projects and projects in Water Science Centers. These quality-control data can be used to assess the quality of microbiological data for the associated environmental samples.
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Data were collected as part of an investigation developed by Leetown Science Center to investigate the comparative detection of avian influenza viruses from waterfowl and potential environmental reservoirs such as aquatic sediment from waterfowl habitat. This dataset identifies positive or negative test results for qRT-PCR (quantitative reverse transcriptase polymerase chain reaction) for avian influenza virus through identification of the Type A influenza virus matrix gene from aquatic sediment samples. Sediment samples were collected from waterfowl habitat so as to determine if temporal and spatial differences in virus detection by qRT-PCR were evident among test sites.
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In 2010, a mass mortality of pufferfish in Hawaii was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that 1) a marine toxin was associated with a kill of a fish species that is, itself, toxic; 2) we provide a plausible mechanism to explain clinical signs of affected fish, and 3) this epizootic likely depleted puffer populations. Whilst our data are compelling, we did not synthesize the toxin de-novo, we were unable to categorically prove that the polar toxins caused mortality or that they were metabolites of an undefined...
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The files in this data release are the processed DNA consensus sequence alignment files for the chelonid herpesvirus 5 (ChHV-5) genome plus 66 open reading frames representing protien-coding genes. DNA was extracted from tumor biopsies from green sea turtles (Chelonias mydas) with fibropapillomatosis sampled from Florida and Hawaii coastal waters. We used high-throughput short-read sequencing (Illumina MiSeq and Ion Torrent Personal Genome Machine) of long-range PCR products amplified from tumor tissue using primers designed from the single available ChHV5 reference genome from a Hawaii green sea turtle. The raw sequence data is available at the NCBI Sequence Read Archive under bioproject number PRJNA360405, including...
Tags: Class Reptilia, DNA sequencing, Earth, Oceans, Atlantic, North Atlantic, Earth, Oceans, Pacific, Family Cheloniidae, All tags...


    map background search result map search result map Puffer Mortality Data Targeted amplicon resequencing of chelonid alphaherpesvirus 5 from tumor samples collected from Chelonia mydas (green turtle) inhabiting Hawaiian and Florida coastal waters Molecular detection of avian influenza virus from sediment samples in waterfowl habitats on the Delmarva Peninsula, USA Laboratory quality-control data associated with samples analyzed for microbiological constituents at the USGS Ohio Water Microbiology Laboratory Puffer Mortality Data Targeted amplicon resequencing of chelonid alphaherpesvirus 5 from tumor samples collected from Chelonia mydas (green turtle) inhabiting Hawaiian and Florida coastal waters Laboratory quality-control data associated with samples analyzed for microbiological constituents at the USGS Ohio Water Microbiology Laboratory