Filters: Tags: metabolites (X)2 results (13ms)
Neonicotinoids have become the most widely used insecticides in world with rapid growth in applications as seed coatings. Nontarget organisms are exposed to concentrated levels of pesticidal active ingredients through ingestion of treated seeds. To better understand pesticide fate, analytical methods are necessary to rapidly screen and accurately quantitate contaminants in environmental and biological matrices. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is commonly employed for neonicotinoid analyses but requires expensive analytical instrumentation and potentially laborious sample preparation. Enzyme-linked immunosorbent assays (ELISAs) are efficient and sensitive alternative methods for neonicotinoid...
Absorption, distribution, metabolism, and elimination of seed-treatment pesticides following the dosing of Japanese quail (Coturnix japonica)
Japanese quail (Coturnix japonica) were orally dosed with pesticide-treated or control-untreated wheat seeds. Pesticide treated wheat seeds were analytically verified for active ingredients of imidacloprid, metalaxyl, tebuconazole, and fludioxonil. Pesticide and metabolite residues were measured in plasma, liver, brain, kidney, muscle, and excreta of exposed and control birds. Samples were extracted via solid phase extraction, pressurized liquid extraction, or sonication. Clean-up steps included protein precipitation followed by solid phase extraction. All samples were analyzed by liquid chromatography tandem mass spectrometry.