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We report the first simultaneous measurements of ?15N and ?13C of DNA extracted from surface soils. The isotopic composition of DNA differed significantly among nine different soils. The ?13C and ?15N of DNA was correlated with ?13C and ?15N of soil, respectively, suggesting that the isotopic composition of DNA is strongly influenced by the isotopic composition of soil organic matter. However, in all samples DNA was enriched in 13C relative to soil, indicating microorganisms fractionated C during assimilation or preferentially used 13C enriched substrates. Enrichment of DNA in 15N relative to soil was not consistently observed, but there were significant differences between ?15N of DNA and ?15N of soil for three...
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In 2015-2016, the Ohio Division of Wildlife’s undercover law enforcement purchased 1,200 grass carp (Ctenopharyngodon idella). Fish heads and eyeballs were sent overnight to U.S. Geological Survey Wetland and Aquatic Research Center for ploidy analysis. Field and laboratory standard operating procedures were established and followed. Fish lengths, fish weights, and eyeball weights were obtained from the U.S. Fish and Wildlife Service’s feral carp ploidy program for grass carp and black carp (Mylopharyngodon piceus) and the Ohio grass carp. Internal 2µm or 4µm bead standards were used in establishing nuclear sizes from Nile tilapia (Oreochromis niloticus), known diploid (n=20) and triploid (n=20) carp blood, as well...
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This dataset contains stream-stage data for Santa Cruz River at Tumacácori Downstream Site (SC11). The pressure transducer was mounted to a metal post secured in the stream near the left bank. The data were recorded using non-vented Solinst Levelogger for the period of June 16, 2016 to June 24, 2016. The data were logged continuously at a 15-minute interval and manually downloaded. Data were then corrected with the atmospheric pressure collected from a Solinst Barologger located at SC14. The data was processed and analyzed for erroneous data.
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This data set describes nuclear microsatellite genotypes derived from six autosomal loci (AgCA222, Age7.1JT, BV13, BV20, NVH206, NVH195-2) in Cooper's Hawks. Samples originated from blood samples collected from live trapped birds. Version History: First release: December 2020 Revised: February 2022 (ver. 2.0)
We report the first simultaneous measurements of ?15N and ?13C of DNA extracted from surface soils. The isotopic composition of DNA differed significantly among nine different soils. The ?13C and ?15N of DNA was correlated with ?13C and ?15N of soil, respectively, suggesting that the isotopic composition of DNA is strongly influenced by the isotopic composition of soil organic matter. However, in all samples DNA was enriched in 13C relative to soil, indicating microorganisms fractionated C during assimilation or preferentially used 13C enriched substrates. Enrichment of DNA in 15N relative to soil was not consistently observed, but there were significant differences between ?15N of DNA and ?15N of soil for three...
We characterized, at millimeter resolution, bacterial biomass, diversity, and vertical stratification of biological soil crusts in arid lands from the Colorado Plateau. Microscopic counts, extractable DNA, and plate counts of viable aerobic copiotrophs (VAC) revealed that the top centimeter of crusted soils contained atypically large bacterial populations, tenfold larger than those in uncrusted, deeper soils. The plate counts were not always consistent with more direct estimates of microbial biomass. Bacterial populations peaked at the immediate subsurface (1-2 mm) in light-appearing, young crusts, and at the surface (0-1 mm) in well-developed, dark crusts, which corresponds to the location of cyanobacterial populations....
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Here we provide public access to six DNA sequence alignments and details on all specimens utilized in Smith and Johnson (2020).
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These are genetic data collected from replicated samples of 21 Enteroctopus (E. dofleini or a cryptic lineage) in Prince William Sound Alaska to evaluate tissue type, DNA extraction method, and time until analyses are completed on data reliability. Data collected from all samples include two microsatellites identified as possible lineage indicators, and nine microsatellite loci previously identified as polymorphic in both lineages. DNA sequence data from 528 bp of the octopine dehydrogenase (OCDE) gene were also collected.
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This dataset contains stream-stage data for Santa Cruz River at Santa Gertrudis Lane (SC10). The pressure transducer was mounted to a metal post secured in the stream near the right bank. The data were recorded using non-vented Solinst Levelogger for the period of February 12, 2015 to December 31, 2016. The data were logged continuously at a 15-minute interval and manually downloaded. Data were then corrected with the atmospheric pressure collected from a Solinst Barologger located at SC14 and analyzed for erroneous data.
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This dataset contains stream-stage data for Santa Cruz River at Anza Trail Crossing (SC12). The pressure transducer was mounted to a metal post secured in the stream near the left bank. The data were recorded using non-vented Solinst Levelogger for the period of May 31, 2016 to December 31, 2016. The data were logged continuously at a 15-minute interval and manually downloaded. Data were then corrected with the atmospheric pressure collected from a Solinst Barologger located at SC14 and analyzed for erroneous data.
The Utah sucker (Catostomus ardens) is endemic to the Bonneville Basin and the upper Snake River drainage in western North America, and is thought to hybridize with the federally endangered June sucker (Chasmistes liorus mictus) in Utah Lake (Bonneville Basin). Here we describe the discovery of a major subdivision in Utah suckers (4.5% mitochondrial sequence divergence) between the ancient Snake River drainage and the Bonneville Basin. This boundary has not previously been recognized in Utah suckers based on morphologic variation, but has been recently described in two endemic cyprinids in the region. Populations in valleys east of the Wasatch Mountains in Utah clustered with the Snake River populations, suggesting...
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This dataset provides cycle threshold (Ct) values achieved during qPCR for 6 sex-linked genes, and two autosomal genes, for individuals from 60 species and across 13 orders of birds.
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This dataset contains stream-stage data for Nogales Wash at Ruby Road (NW8). The pressure transducer was mounted to a metal post secured in the stream near the left bank. The data were recorded using non-vented Solinst Levelogger for the period of February 12, 2015 to December 31, 2016. The data were logged continuously at a 15-minute interval and manually downloaded. Data were then corrected with the atmospheric pressure collected from a Solinst Barologger located at SC14 and analyzed for erroneous data.
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These data are comprised of two tables, one table containing wolverine (Gulo gulo) sample and genetic information for 20 microsatellite loci (Gg10-1, Gg25, Gg37-2, Gg42-1, Gg192-1, Gg443, Gg452, Gg454, Gg465, Gg471, Gg473, Gg-3-1, Gg-4, Gg-7-1, Ggu_216-1, Lut604, Ma-3-1, Mvis075, Tt-1, and Tt-4) and genetic sex determination results from North America and one Russian wolverine. All samples were obtained from collections at the University of Alaska Museum of the North or the University of New Mexico’s Museum of Southwestern Biology. The second table provides complete primer information for the genetic markers since several were redesigned from their originally-published sequences for this study.
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This dataset contains E. coli data collected at the Outfall of the Nogales International Wastewater Treatment Plant (NIWTP; SC6, 312724110580501) by U.S. International Boundary and Water Commission (IBWC) personnel. The IBWC collects discrete samples at the Outfall of the NIWTP daily or weekly by dipping a 100 mL sterilized IDEXX bottle in the centroid of flow. Samples are kept chilled and shipped to Turner Laboratories Inc. or Legend Technical Services Inc. Both laboratories use standard method 9223B for E. coli analysis, which uses the Colilert™-24 (Colilert) and Quanti-Tray®/2000 (Quanti-Tray) system. The holding time specified for wastewater is 30 hours. The dataset is a compilation of multiple paper records....
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Conclusions: Grizzly bear population fragmentation corresponded to the presence of settled mountain valleys and major highways. In these disturbed areas, the inter-area movements of female bears was affected more than for male bears. Without female connectivity, small subpopulations of grizzly bears are not viable over the long term. Thresholds/Learnings: Females grizzlies reduced their movement rates drastically when settlement increased to >20% of a given area. In highly settled areas (>50% settlement), both sexes demonstrated similar reductions in movement. Synopsis: Researchers studied the current state and potential causes of population fragmentation in grizzly bears over western Canada, the Greater Yellowstone...
Categories: Publication; Types: Citation, Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Alberta, British Columbia, Canadian Rockies, Chilcotin Ranges and Fraser Plateau, Clear Hills and Western Alberta Upland, All tags...
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In this dataset offspring are assigned to a dam and sire using information from single nucleotide polymorphisms (SNPs) from previously published pedigree reassignments of Brown Treesnakes within the U.S. Geological Survey's Brown Treesnake study enclosure on Northwest Field of Andersen Air Force Base, Guam (Levine and Yackel Adams 2021 and Nafus 2021) to update the overall pedigree dataset for snakes (2009-2018). Genomic pedigree reconstruction can assign offspring to parents in invasive species for which mating, and reproduction are difficult to study.
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This dataset contains stream-stage data for the Santa Cruz River near Nogales International Wastewater Treatment Plant Upstream of Railroad Trestle (SC5). The pressure transducer was mounted to a metal post secured in the stream near the right bank. The data were recorded using non-vented Solinst Levelogger for the period of February 12, 2015 to December 31, 2016. The data were logged continuously at a 15-minute interval and manually downloaded. Data were corrected with the atmospheric pressure collected from a Solinst Barologger located at SC14 and checked for erroneous data.
Although cyclosporine has high specificity for the immune system, immunosuppressive therapy with CsA is often complicated by nephrotoxicity. The main morphologic targets of CsA nephrotoxicity include the tubular epithelial and endothelial cells. These cells were investigated in vitro. CsA caused a dose- and time-dependent inhibition of cell growth, vacuolization and fatty change in adherent cells, detachment, and cell death. Inhibition of 3H-TdR incorporation in cells of both tubular epithelial and endothelial origin occurred between 3 microM and 10 microM. Electron microscopy studies revealed cellular swelling, dilatation of the endoplasmic reticulum, and the presence of lipid droplets and giant mitochondria. The...
map background search result map search result map Population fragmentation and inter-ecosystem movements of grizzly bears in western Canada and the northern United States. Stream-stage Dataset at Nogales Wash at Ruby Road, geographic NAD83 (Water Years 2015–2017) Stream-stage Dataset at Santa Cruz River at Anza Trail Crossing, geographic NAD83 (Water Years 2015–2017) Stream-stage Dataset at Santa Cruz River at Santa Gertrudis Lane, geographic NAD83 (Water Years 2015–2017) Stream-stage Dataset at Santa Cruz River at Tumacacori Downstream Site, geographic NAD83 (Water Year 2016) Stream-stage Dataset at Santa Cruz River near Nogales International Wastewater Treatment Plant Upstream of Railroad Trestle, geographic NAD83 (Water Years 2015–2017) E. coli dataset at from the Outfall of the Nogales International Wastewater Treatment Plant, geographic NAD83 (Water Years 2009–2015) Enteroctopus Sampling Effects on Genetic Data, Prince William Sound, Alaska, 2012-2015 Establishing a standard ploidy assessment method using grass carp from Ohio, 2015-2016 Novel genetic resources to facilitate future molecular studies in freshwater mussels (Bivalvia: Unionidae) Molecular data to investigate phylogeographic patterns, species boundaries, and demographic history of a North American freshwater mussel species complex (Bivalvia: Unionidae) Offspring, dam, sire pedigree assignments in a managed population of Brown Treesnakes on Guam Genetic Data from Wolverine (Gulo gulo) of North America Genetic Data from Cooper's Hawks (Accipiter cooperii), North America Offspring, dam, sire pedigree assignments in a managed population of Brown Treesnakes on Guam Stream-stage Dataset at Nogales Wash at Ruby Road, geographic NAD83 (Water Years 2015–2017) Stream-stage Dataset at Santa Cruz River at Anza Trail Crossing, geographic NAD83 (Water Years 2015–2017) Stream-stage Dataset at Santa Cruz River at Tumacacori Downstream Site, geographic NAD83 (Water Year 2016) Stream-stage Dataset at Santa Cruz River near Nogales International Wastewater Treatment Plant Upstream of Railroad Trestle, geographic NAD83 (Water Years 2015–2017) E. coli dataset at from the Outfall of the Nogales International Wastewater Treatment Plant, geographic NAD83 (Water Years 2009–2015) Stream-stage Dataset at Santa Cruz River at Santa Gertrudis Lane, geographic NAD83 (Water Years 2015–2017) Enteroctopus Sampling Effects on Genetic Data, Prince William Sound, Alaska, 2012-2015 Establishing a standard ploidy assessment method using grass carp from Ohio, 2015-2016 Novel genetic resources to facilitate future molecular studies in freshwater mussels (Bivalvia: Unionidae) Genetic Data from Cooper's Hawks (Accipiter cooperii), North America Population fragmentation and inter-ecosystem movements of grizzly bears in western Canada and the northern United States. Genetic Data from Wolverine (Gulo gulo) of North America Molecular data to investigate phylogeographic patterns, species boundaries, and demographic history of a North American freshwater mussel species complex (Bivalvia: Unionidae)