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Accurate assessment of species identity is fundamental for conservation biology. Using molecular markers from the mitochondrial and nuclear genomes, we discovered that many putatively native populations of greenback cutthroat trout (Oncorhynchus clarkii stomias) comprised another subspecies of cutthroat trout, Colorado River cutthroat trout (Oncorhynchus clarkii pleuriticus). The error can be explained by the introduction of Colorado River cutthroat trout throughout the native range of greenback cutthroat trout in the late 19th and early 20th centuries by fish stocking activities. Our results suggest greenback cutthroat trout within its native range is at a higher risk of extinction than ever before despite conservation...
Populations of the freshwater mussel genus Anodonta appear to be in a state of rapid decline in western North America, following a trend that unfortunately seems to be prevalent among these animals (Mollusca: Unionoida). Here we describe the patterns of molecular divergence and diversity among Anodonta populations in the Bonneville Basin, a large sub-basin of the Great Basin in western North America. Using amplified fragment length polymorphism (AFLP) analysis, we found a striking lack of nuclear diversity within some of these populations, along with a high degree of structuring among populations (FST = 0.61), suggesting post-Pleistocene isolation, due either to a long-term loss of hydrologic connectivity among...
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Prior to removal of pest species from an area, resource managers must determine if re-immigration from another population is possible. Voles inhabiting Saddle Rock on the southern Oregon coast are suspected to be partially responsible for declines in the Leach's storm petrel colony on the island. The island is very close to the mainland, and it is potentially accessible during below-average low tides. USGS scientists Mark Miller and Susan Haig and colleagues used genetic techniques to assess island vole population connectivity.
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Single sequence repeat (SSR) and amplified fragment length polymorphic (AFLP) molecular marker genotypes in cheatgrass (Bromus tectorum) were compared to published data on phenotypic variation in seed dormancy, vernalization requirement, and resistance to the pathogen Ustilago bullata. Several features of cheatgrass facilitated this study: it is a recent invader in the western United States, has considerable phenotypic polymorphism, and is an obligate self-pollinator. Forty self-pollinating lines from four populations common to the three phenotypic data sets were analyzed for molecular genetic variation using seven SSR loci and 31 AFLP loci. We examined correlations between distance matrices using the Mantel test...
    map background search result map search result map Correlation between molecular markers and adaptively significant genetic variation in Bromus tectorum (Poaceae), an inbreedingannual grass Across the great divide: genetic forensics reveals misidentification of endangered cutthroat trout populations. Amplified Fragment Length Polymorphism (AFLP) data obtained for 34 long-tailed vole (Microtus longicaudus) individuals at 91 loci Amplified Fragment Length Polymorphism (AFLP) data obtained for 34 long-tailed vole (Microtus longicaudus) individuals at 91 loci Across the great divide: genetic forensics reveals misidentification of endangered cutthroat trout populations. Correlation between molecular markers and adaptively significant genetic variation in Bromus tectorum (Poaceae), an inbreedingannual grass