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Resource agencies are searching for effective methods to prevent the spread of invasive Silver Carp (Hypophthalmichthys molitrix) and Bighead Carp (Hypophthalmichthys nobilis), hereafter bigheaded carps, from the Mississippi River basin into the Laurentian Great Lakes. Elevating carbon dioxide (CO2) concentrations in water within locks and other pinch points is an approach being considered to reduce invasive fish passage. Laboratory studies have shown that bigheaded carps strongly avoid areas of elevated CO2 (Kates et al. 2012; Dennis et al. 2015). Similarly, telemetry studies found that CO2 can be used to exclude bigheaded carps from certain locations (Donaldson et al. 2016) and reduce upstream movement (Cupp et...