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Summary Non-point nitrogen (N) and phosphorus (P) pollution from agriculture has increasingly received more public attention. In this study, NO3–N, dissolved P (DP) and particulate P (PP) concentrations and loads were investigated for four sub-basins (labeled 1–4 going up the watershed) within a mixed land use watershed (39.5 ha) in the Appalachian Valley and Ridge Physiographic Province. The hot moments of NO3–N concentration and load occurred in base flow and during the non-growing season. Great and temporally variable DP and PP concentrations were observed in storm flow. The hot moments of DP concentration and load were in storm flow from May to December and from September to Nov, respectively, while the hot...