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Echinoderms such as urchins are important in marine ecosystems, particularly as grazers, and unhealthy urchins can have important ecological implications. For instance, unexplained mortalities of Diadema sp. in the Caribbean were followed by algal overgrowth and subsequent collapse of coral reef ecosystems. Unfortunately few tools exist to evaluate echinoderm health making management of mortalities or other health issues problematic. Hematology is often used to assess animal health in many animal groups including invertebrates but is seldom applied to echninoderms. We used a standard gravitometric technique to concentrate fixed ceolomocytes from the collector urchin Tripneustes gratilla onto microscope slides permitting...
Sea star wasting disease (SSWD) is a suite of poorly described non-specific clinical signs including abnormal posture, epidermal ulceration, and limb autotomy (sloughing) causing mortalities of over 20 species of sea stars and subsequent ecological shifts throughout the northeastern Pacific. While SSWD is widely assumed to be infectious with environmental conditions facilitating disease progression, little data exist on cellular changes associated with disease that could inform mechanisms of disease pathogenesis and host susceptibility. Here, we replicated SSWD by exposing captive Pisaster ochraceus to a suite of non-infectious organic substances and show that development of gross lesions is a basal to surface process...
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Here we compare pollen identification results derived from light microscopy and DNA sequencing techniques of a robust number of samples collected from honey bee colonies embedded within intensive agricultural landscapes in the Northern Great Plains. We collected pollen samples from colonies within 6 apiaries in 2010 and 2011. For each pollen sample, we identified pollen grains via light microscopy and provide the number of grain counts-per-million. A separate aliquot of each pollen sample subjected to light microscope identification was also used for DNA sequencing analysis. We provide the plant operational taxonomic unit (OTU) for all base pair reads as the number of reads-per-million.


    map background search result map search result map A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding datasets Data on blood cells of the collector urchin, Tripneustes gratilla Sea star wasting disease pathology Data on blood cells of the collector urchin, Tripneustes gratilla A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding datasets