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The presence or absence of heterozygous individuals and overall genetic variation were determined for the invasive exotic annual grass Bromus tectorum from four populations in northern Nevada using seven species-specific polymorphic di-nucleotide microsatellite markers. Allelic polymorphisms were found in all populations, but not at all loci within each population. Mean genetic diversity across loci within populations ranged from 0.009 ± 0.006 to 0.551 ± 0.073, across populations x̄ = 0.234 ± 0.043. Fifteen individuals from the total sample (8.24%) were heterozygous at 1–4 loci. Two populations exhibited no heterozygosity and the remaining two populations had relative heterozygote proportions of 22.5% and...