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The goal of this project was to quantify the background physiological condition of mussels in the Minnesota and St. Croix River basins using glycogen as an indicator of physiological stress. We obtained 230 samples of foot tissue from Lampsilis cardium (n=118) and Lasmigona complanata (n=112) from a total of four rivers during September 1-5, 2015. From each sample, we weighed out a 10.0 ± 2.0 mg aliquot for glycogen determination by the alkaline digestion and phenol-sulfuric acid spectrophotometric method. The accuracy of glycogen determinations was quantified by the use of procedural blanks, replicates of an in-house reference material, triplicate analysis of four aqueous calibration standards, duplicate analysis...
To quantify the responses of native mussel to the range of environmental conditions in the Minnesota River and St. Croix River basins, laboratory experiments will examine the physiological responses of mussels to different levels of suspended sediment and bed instability over a range of flow rates. Experiments will be conducted in the Outdoor StreamLab and flumes at the St. Anthony Falls Laboratory at the University of Minnesota. The experimental streams allow for direct manipulation of environmental conditions in a field-scale channel while collecting laboratory quality measurements. Laboratory experiments will focus on mussel response to suspended sediment (water quality) and bed instability (increased flows or...