Filters: Tags: genotype (X)19 results (84ms)
Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus
Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.
On August 25, 2015 speaker Matt Germino presented on his work restoring sagebrush in the Great Basin. Shrubs are ecosystem foundation species in most of the Great Basin’s landscapes. Most of the species, including sagebrush, are poorly adapted to the changes in fire and invasive pressures that are compounded by climate change. This presentation gives an overview of challenges and opportunities regarding restoration of sagebrush and blackbrush, focusing on climate adaptation, selection of seeds and achieving seeding and planting success. Results from Great Basin LCC supported research on seed selection and planting techniques are presented.
Genotype Data for Eastern Massasauga Rattlesnakes (Sistrurus catenatus) from Bois Blanc Island, Michigan at 15 Microsatellite DNA Loci
We investigated fine-scale genetic patterns of the federally threatened Eastern Massasauga Rattlesnake (Sistrurus catenatus) on a relatively undisturbed island in northern Michigan, USA. This species often persists in habitat islands throughout much of its distribution due to extensive habitat loss and distance-limited dispersal. These data are from 102 individual Eastern Massasauga Rattlesnakes sampled at Bois Blanc Island, Michigan and genotyped at 15 microsatellite loci. Samples were collected as part of a study to examine functional connectivity for the Eastern Massasauga. We found that the entire island population exhibited weak genetic structuring with spatially segregated variation in effective migration...
Effects of fungal endophytes on invasive Phragmites australis (ssp. australis) performance in growth chamber and field experiments
These data tables contain data collections from field experiments of Phragmites australis (ssp. australis) treated with known fungal endophytes. Tiller counts, tiller diameter, and tiller height measurements were taken every two weeks over an eight-week study period. Clones of Phragmites plants were collected from three different locations: Sandusky, Michigan; Bloomington, Indiana; and the Ottawa National Wildlife Refuge near Oak Harbor, Ohio. Additionally, data collections from a similar experiment of Phragmites australis (ssp. australis) treated with known fungal endophytes performed in a growth chamber are included. Tiller numbers and tiller heights were measured every three weeks over 15 total weeks for the...
Here we provide public access to UCE DNA sequences for all genetic samples in Byerly et al. 2022b.
Effects of Genotype and Management Treatments of Native and Invasive Herbs on Success of Sagebrush Restoration
FY2013The increase in large wildfires at a time when habitat for Greater Sage Grouse and other species dependent on big sagebrush has also increased has led to substantial needs for big sagebrush seeds. Significant decisions on which sagebrush seed to use and on management treatments that affect competing herb layers on the same restoration sites affect the trajectory of habitat.This project will evaluate how seed source, specifically genotype and climate-of-origin, interact with landscape-scale and replicated treatments (fencing, herbicide application, mowing, and seeding).
Microsatellite genotypes for Lake Sturgeon (Acipenser fulvescens) Eggs and Larvae from Constructed Reefs in the St. Clair-Detroit River System (2015-2016)
This dataset contains the physical collection information (e.g., sample location, date, gear type) and microsatellite DNA genotype of egg and larval Lake Sturgeon collected in the St. Clair and Detroit rivers in 2015 and 2016. Individuals were genotyped for 18 microsatellite loci (13 disomic and 5 polysomic). Alleles (base pair sizes) were recorded as presence absence scores (1:present, 2:absent, 0:missing data) for all previously observed alleles. Thus individual genotypes were recorded as pseudo diploid dominant phenotypes resulting in individual vectors of length n=205 for each genotyped egg or larval individual.
In 2013, the U.S. Fish and Wildlife Service (USFWS) Conservation Objectives Team (COT) identified wildfire and the associated conversion of low- to mid-elevation sagebrush (Artemisia tridentata) habitats to invasive annual grass-dominated vegetation communities as the two primary threats to the sustainability of Greater sage-grouse (Centrocercus urophasianus, hereafter GRSG) in the western portion of the species range (USFWS 2013). To facilitate the examination and evaluation of the role fire and invasive plants play in the conservation of GRSG, the USFWS solicited the assistance of the Western Association of Fish and Wildlife Agencies (WAFWA) to conduct a collaborative assessment of the conservation challenges...
These data show the multilocus genotypes, gender, and midline carapace length (MCL) for desert tortoises (Gopherus agassizii) sampled in the central portion of the Mojave desert tortoise range. This data supports the following publication: Dutcher, K.E., Vandergast, A.G., Esque, T.C., Mitelberg, A., Matocq, M.D., Heaton, J.S. and Nussear, K.E., Genes in space: what Mojave Desert tortoise genetics can tell us about landscape connectivity. Conservation Genetics, pp.1-15.
Microsatellite Genetic Marker Genotypes from Southern Mule Deer (Odocoileus hemionus fuliginatus) Sampled in San Diego County, California
Collection of microsatellite genetic data from multiple projects involving southern mule deer (Odocoileus hemionus fuliginatus) in San Diego County, California. Samples were collected 2005-2007, 2010, 2012-2013, 2015, and 2018-2020. These data support the following publication: Mitelberg, A., Smith, J.G., and Vandergast, A.G., 2019, DNA Fingerprinting of Southern mule deer (Odocoileus hemionus fuliginatus) in north San Diego County, California (2018–19): U.S. Geological Survey Open-File Report 2019–1138, 25 p., https://doi.org/10.3133/ofr20191138.
Two independent sampling methods were used to collect genetic samples from grizzly bears (Ursus arctos): (i) hair traps—corrals of barbed wire with lure in the center systematically distributed using an 8 x 8 km (1998, 2000) or 7 x 7 km (2004) grid and (ii) bear rubs—naturally occurring trees or other objects that bears rub on fitted with barbed wire (1998–2000, 2004, and 2009–2012). From 1998 to 2000, sampling occurred on 8000 km2 in the northern extent of the Northern Continental Divide Ecosystem (NCDE), whereas systematic and consistent, ecosystem-wide sampling occurred in 2004 and 2009–2012. In total, there were 6160 confirmed grizzly bear detections, leading to the identification of 1115 unique individual genotypes...
Historical genetic resources for Northwestern Atlantic and Caribbean populations of Roseate Tern Sterna dougallii, 1879-1940
Here we provide public access to UCE DNA sequences for all genetic samples in Byerly et al. 2022.
Nuclear microsatellite genotypes of six populations of yellow rail (Coturnicops noveboracensis) sampled 2005-2008
These data reflect nuclear microsatellite genotypes for specimens of yellow rails (Coturnicops noveboracensis) sampled in 2005-2008. Data from six populations are included.
These data show the multilocus genotypes, as well as extraction and sample genotype quality assessments for desert tortoise (Gopherus agassizii) scat samples. These data support the following publication: Mitelberg, A., Vandergast, A.G., Nussear, K.E., Dutcher, K. and Esque, T.C., 2019. Development of a Genotyping Protocol for Mojave Desert Tortoise Scat. Chelonian Conservation and Biology. https://doi.org/10.2744/CCB-1394.1.
Arrowweed (Pluchea sericea) morphological and physiological response data from a greenhouse inundation experiment
These data were collected to evaluate if arrowweed (Pluchea sericea) individuals and genotypes collected from across a 5.3 degree Celsius temperature gradient and grown in a common greenhouse environment had different physiological and morphological responses to inundation. The objectives of the study were to assess if 1) individuals from climatically different provenances differ in their morphological and physiological phenotypes, 2) individuals from climatically different provenances differ in their response to inundation, and 3) phenotypes and inundation responses are partially controlled by genotype. These data represent measurements made on 248 plants that were grown in a greenhouse and, also in a greenhouse...
This data set describes nuclear microsatellite genotypes derived from eleven autosomal loci (Lar24, Lar12, Lar26, Lar19, Rbg13, K16, Rbg18, Hg18, Hg16, Hg25, Rbg29). A total of 651 Larus gulls were examined for this study with samples collected throughout North America, Europe, Russia, and South America.
Microsatellite Genotypes for Coastal Cactus Wrens (Campylorhynchus brunneicapillus) from Southern California, 2009-2019
These data show multilocus genotypes, banding age, and territory for coastal cactus wrens (Campylorhynchus brunneicapillus) sampled in coastal southern California between 2009 and 2019. These data support the following publication: Vandergast, A.G., Kus, B.E., Smith, J.G. and Mitelberg, A., 2022. Recent declines in genetic diversity with limited dispersal among coastal cactus wren populations in San Diego County, California. Conservation Science and Practice, 4(9), p.e12780. https://doi.org/10.1111/csp2.12780.
Novel genetic resources for Clubshell freshwater mussels (Pleurobema clava, P. oviforme) for enhanced conservation
Here we provide public access to two mitochondrial DNA sequence alignments, multi-locus microsatellite DNA genotypes, morphometric measurements and details on all specimens utilized in Morrison et al., 2021 (In press).
Mitochondrial and microsatellite genetic data from Cape Sable seaside sparrow feather samples collected 2011-2018
The federally endangered Cape Sable seaside sparrow (Ammospiza maritima mirabilis) is endemic to the Greater Everglades ecosystem in southern Florida. The subspecies inhabits the fragmented freshwater marl prairies and contains six subpopulations (A-F), named for management purposes, since genetic information is lacking. To evaluate genetic connectivity and inform management decisions, feather samples were collected between 2011 and 2018 from 108 sparrows across five subpopulations (A-E). Sequence data for four mitochondrial DNA markers (N = 36–69) and 12 nuclear microsatellite loci (N = 55) are provided. The mitochondrial markers used were Cytochrome b (Cytb), control region D-loop, NADH dehydrogenase 2 (ND2),...