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On August 25, 2015 speaker Matt Germino presented on his work restoring sagebrush in the Great Basin. Shrubs are ecosystem foundation species in most of the Great Basin’s landscapes. Most of the species, including sagebrush, are poorly adapted to the changes in fire and invasive pressures that are compounded by climate change. This presentation gives an overview of challenges and opportunities regarding restoration of sagebrush and blackbrush, focusing on climate adaptation, selection of seeds and achieving seeding and planting success. Results from Great Basin LCC supported research on seed selection and planting techniques are presented.
Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus
Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.
Effects of Genotype and Management Treatments of Native and Invasive Herbs on Success of Sagebrush Restoration
FY2013The increase in large wildfires at a time when habitat for Greater Sage Grouse and other species dependent on big sagebrush has also increased has led to substantial needs for big sagebrush seeds. Significant decisions on which sagebrush seed to use and on management treatments that affect competing herb layers on the same restoration sites affect the trajectory of habitat.This project will evaluate how seed source, specifically genotype and climate-of-origin, interact with landscape-scale and replicated treatments (fencing, herbicide application, mowing, and seeding).
Effects of fungal endophytes on invasive Phragmites australis (ssp. australis) performance in growth chamber and field experiments
These data tables contain data collections from field experiments of Phragmites australis (ssp. australis) treated with known fungal endophytes. Tiller counts, tiller diameter, and tiller height measurements were taken every two weeks over an eight-week study period. Clones of Phragmites plants were collected from three different locations: Sandusky, Michigan; Bloomington, Indiana; and the Ottawa National Wildlife Refuge near Oak Harbor, Ohio. Additionally, data collections from a similar experiment of Phragmites australis (ssp. australis) treated with known fungal endophytes performed in a growth chamber are included. Tiller numbers and tiller heights were measured every three weeks over 15 total weeks for the...
In 2013, the U.S. Fish and Wildlife Service (USFWS) Conservation Objectives Team (COT) identified wildfire and the associated conversion of low- to mid-elevation sagebrush (Artemisia tridentata) habitats to invasive annual grass-dominated vegetation communities as the two primary threats to the sustainability of Greater sage-grouse (Centrocercus urophasianus, hereafter GRSG) in the western portion of the species range (USFWS 2013). To facilitate the examination and evaluation of the role fire and invasive plants play in the conservation of GRSG, the USFWS solicited the assistance of the Western Association of Fish and Wildlife Agencies (WAFWA) to conduct a collaborative assessment of the conservation challenges...
Microsatellite genotypes for Lake Sturgeon (Acipenser fulvescens) Eggs and Larvae from Constructed Reefs in the St. Clair-Detroit River System (2015-2016)
This dataset contains the physical collection information (e.g., sample location, date, gear type) and microsatellite DNA genotype of egg and larval Lake Sturgeon collected in the St. Clair and Detroit rivers in 2015 and 2016. Individuals were genotyped for 18 microsatellite loci (13 disomic and 5 polysomic). Alleles (base pair sizes) were recorded as presence absence scores (1:present, 2:absent, 0:missing data) for all previously observed alleles. Thus individual genotypes were recorded as pseudo diploid dominant phenotypes resulting in individual vectors of length n=205 for each genotyped egg or larval individual.
These data show the multilocus genotypes, gender, and midline carapace length (MCL) for desert tortoises (Gopherus agassizii) sampled in the central portion of the Mojave desert tortoise range. This data supports the following publication: Dutcher, K.E., Vandergast, A.G., Esque, T.C., Mitelberg, A., Matocq, M.D., Heaton, J.S. and Nussear, K.E., Genes in space: what Mojave Desert tortoise genetics can tell us about landscape connectivity. Conservation Genetics, pp.1-15.
Two independent sampling methods were used to collect genetic samples from grizzly bears (Ursus arctos): (i) hair traps—corrals of barbed wire with lure in the center systematically distributed using an 8 x 8 km (1998, 2000) or 7 x 7 km (2004) grid and (ii) bear rubs—naturally occurring trees or other objects that bears rub on fitted with barbed wire (1998–2000, 2004, and 2009–2012). From 1998 to 2000, sampling occurred on 8000 km2 in the northern extent of the Northern Continental Divide Ecosystem (NCDE), whereas systematic and consistent, ecosystem-wide sampling occurred in 2004 and 2009–2012. In total, there were 6160 confirmed grizzly bear detections, leading to the identification of 1115 unique individual genotypes...
Historical genetic resources for Northwestern Atlantic and Caribbean populations of Roseate Tern Sterna dougallii, 1879-1940
Here we provide public access to UCE DNA sequences for all genetic samples in Byerly et al. 2022.
These data show the multilocus genotypes, as well as extraction and sample genotype quality assessments for desert tortoise (Gopherus agassizii) scat samples. These data support the following publication: Mitelberg, A., Vandergast, A.G., Nussear, K.E., Dutcher, K. and Esque, T.C., 2019. Development of a Genotyping Protocol for Mojave Desert Tortoise Scat. Chelonian Conservation and Biology. https://doi.org/10.2744/CCB-1394.1.
Novel genetic resources for Clubshell freshwater mussels (Pleurobema clava, P. oviforme) for enhanced conservation
Here we provide public access to two mitochondrial DNA sequence alignments, multi-locus microsatellite DNA genotypes, morphometric measurements and details on all specimens utilized in Morrison et al., 2021 (In press).
Microsatellite Genotypes for Coastal Cactus Wrens (Campylorhynchus brunneicapillus) from Southern California, 2009-2019
These data show multilocus genotypes, banding age, and territory for coastal cactus wrens (Campylorhynchus brunneicapillus) sampled in coastal southern California between 2009 and 2019. These data support the following publication: Vandergast, A.G., Kus, B.E., Smith, J.G. and Mitelberg, A., 2022. Recent declines in genetic diversity with limited dispersal among coastal cactus wren populations in San Diego County, California. Conservation Science and Practice, 4(9), p.e12780. https://doi.org/10.1111/csp2.12780.