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Among the data required by the U.S. Food and Drug Administration (FDA) for approval of an aquaculture drug are the data that characterize the depletion of a drugs marker residue from the edible fillet tissue of fish after exposure. Eugenol is the marker residue for AQUI-S 20E, a product proposed for use as a sedative for fish.Rainbow trout (Onchorynchus mykiss; a representative cold water fish species) was exposed to AQUI-S 20E in water at a temperature of 9 C, a temperature that is representative of the lower range of temperatures where rainbow trout would be sedated. Eighty fish were exposed to a nominal AQUI-S 20E concentration of 100 mg/L for 60 min. Groups of 16 fish were sampled immediately after 60 min of...