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(i) This dataset presents macroscopic and microscopic pathological findings and bacteriology of 178 tadpoles diagnosed with Severe Perkinsea Infections. (ii) Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (iii) Necropsies and gross evaluation of carcasses were carried out under a dissecting microscope. (iv) Severe Perkinsea Infection was confirmed in each specimen by histological observation of pathological changes in tissues with presence of Perkinsea-like organisms in at least one organ, including brain, eyes, gastrointestinal tract, gills, heart, liver, lungs, kidney,...
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Raw sequencing data as generated by the five different methods used are provided for each of the three samples used in the comparison. The files are in FASTQ format as exported from the Oxford Nanopore’s MK1C using MinION flowcells. Files are labeled according to the method (as described in the paper) and the Sample ID). The MK1C exports data in blocks of 6000 reads per FASTQ file and all the FASTQ files from each method and sample are grouped in a common folder.
Results of a literature search performed December 1, 2020. The tables in this document summarize the publications fitting in this search criteria and are split into the publications that update the list of international species that could carry Bsal (Appendix 1 Table S1). Number of individual live amphibians imported in categories of urodelan (salamander, newt, and related taxa) species restricted under the interim regulations and not listed, species not listed on the regulations that have had Bsal detected, notably Bombina spp., anuran species in the genus Rana, and Xenopus (including African clawed frogs) as a comparison of species not thought to be susceptible to Bsal, but commonly imported (Appendix 3 Table...


    map background search result map search result map Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data Evaluating regulations and surveillance as risk-mitigation to the emerging amphibian pathogen Bsal- Data release Raw MinION FASTQ datafiles corresponding to the paper “A comparison of avian influenza virus whole genome sequencing approaches using nanopore technology” Raw MinION FASTQ datafiles corresponding to the paper “A comparison of avian influenza virus whole genome sequencing approaches using nanopore technology” Evaluating regulations and surveillance as risk-mitigation to the emerging amphibian pathogen Bsal- Data release Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data