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Recent concern for the adverse effects from neonicotinoid insecticides has centered on risk for insect pollinators in general and bees specifically. However, natural resource managers are also concerned about the risk of neonicotinoids to conservation efforts for the monarch butterfly (Danaus plexippus) and need a tool to estimate risk for wild monarch butterflies exposed to clothianidin. In the present study, monarch butterfly larvae were exposed to contaminated milkweed plants from hatch until pupation, and the effects upon larval survival, larval growth, pupation success, and adult mass were measured. Soils dosed with a granular insecticide product lead to average clothianidin concentrations of 10.8 - 843 ng/g...
This dataset reports select histopathological changes in liver, kidney, gonad, spleen, brain, heart, skeletal muscle, lung, or adrenal gland of zebra finches exposed to 15mg/kg, 18mg/kg, 30mg/kg, 60mg/kg, 75mg/kg, 125mg/kg, or 250mg/kg clothianidin (CTD) (with controls) by gavage, in water, or on millet seed either as a single dose or for 7 days. The goal of the project is to develop a subacute neonicotinoid toxicity model using zebra finches as a model passerine. We have completed two acute toxicity trials using CTD insecticide. Based on these studies, we estimate the no observable effect level (NOAEL) and the lowest observable effect level (LOAEL) for CTD in zebra finches to be 15 and 30 mg/kg body weight, respectively,...
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Xenopus laevis tadpoles were held in 4 replicate vivaria for each of 4 treatments of neonicotinoid pesticides and one control treatment for at least one month (Control media, thiamethoxam high concentration of 100 ppm, thiamethoxam low concentrations of 20 ppm, clothianidin high concentrations of 100 ppm, and clothianidin low concentration of 20 ppm). Water was sampled for chemical testing on Day 33. Between Day 1 and 44, instantaneous measures of length were collected on a random selection of tadpoles from each vivarium. On Day 44, tadpole length (mm), weight, and developmental stage (Nieuwkoop-Faber staging) were measured for all tadpoles in all vivaria (three of the measured individuals from each replicate...
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Neonicotinoids, a widely used class of insecticide, have been found in surface waters globally. They pose a risk to non-target species found in aquatic environments such as aquatic macroinvertebrates. To better understand the effects of the neonicotinoids imidacloprid, clothianidin and their mixtures on aquatic communities we ran a 30 day mesocosm test. Rock trays were colonized with natural benthic communities in the Cache La Poudre River, located in the mountains of Northern Colorado, and relocated to a laboratory experimental stream setting. In total there were 33 experimental streams: 3 controls and 30 treatments consisting of both single compound and binary compound exposures with 5 treatment levels for each...


    map background search result map search result map Xenopus metamorphosis after neonicotinoid exposure Uptake and toxicity of clothianidin to monarch butterflies from milkweed consumption (ver. 2.0, January 2020) Data set for a mesocosm to field assessment of the ecological risks associated with neonicotinoids in U.S. streams Uptake and toxicity of clothianidin to monarch butterflies from milkweed consumption (ver. 2.0, January 2020) Xenopus metamorphosis after neonicotinoid exposure Data set for a mesocosm to field assessment of the ecological risks associated with neonicotinoids in U.S. streams