Filters: Tags: cell culture (X)
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The dwarf wedgemussel (Alasmidonta heterodon) is a federally endangered freshwater mussel that once ranged from New Brunswick to North Carolina, but now only exists in isolated populations throughout its diminished range. Laboratory propagation in conjunction with augmentation or reintroduction is considered a critical component of dwarf wedgemussel restoration. The presented data aimed to I) develop in-vitro propagation techniques including methods to minimize microbial contamination for two Alasmidonta species and II) compile and assess critical fish assemblage information at existing dwarf wedgemussel presence and absence locations to inform future restoration activities. Results from this project will be used...
Many freshwater mussel species are critically imperiled, and propagation is essential for species‘ recovery. Fungal contamination can negatively affect in vitro propagation of freshwater mussels; thus, we investigated methods of mitigating fungal contamination. Specifically, we tested the effect of medium replacement frequency, antifungal (Amphotericin B) concentrations, and antifungal addition method on risk of fungal contamination and transformation success of two species, Alasmidtonta heterodon and congener, Alasmidonta undulata. We found that contamination risk was higher in treatment groups that were replenished with Amphotericin B every one or two days (vs. every three days), but contamination risk was not...
Categories: Data;
Tags: Massachusetts,
New England,
USGS-EMA-LOW-SB SMC: Freshwater mussels,
amphotericin B,
biota,
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