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Thirty-four species of nematodes were found in 415 Anseriformes (Anatidae) of 27 species; 93.7% of birds over 4 weeks old were infected. Data on prevalence, host specificity, age of host, and geographic distribution are given. Infections were more intense in sick birds and birds in poor physical condition. Accidental or abnormal infection was more likely in sick than in normal birds. From 1 to 13 species of nematodes are reported from each host species, including 118 new host records, 3 nematodes new for North America, and 1 new species. Multiple infections were present in 76.5% of birds parasitized; eight species of nematodes were found in one whistling swan (Olor columbianus).
Categories: Publication; Types: Citation; Tags: Wildlife Disease
This dataset consists of measurements of the digestive gland in the freshwater snail Lymnaea stagnalis orally exposed to uranium. The objective of the study was to assess the morphological effects of uranium on the snail digestive gland by light microscopy. Laboratory-cultured L stagnalis were exposed to either synthetic or natural solid phases of uranium and a subset (6 controls and 6 exposed) were euthanized and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). The size of each sample, the length and width of digestive gland tubules (5 random glands per sample) and height of digestive cells...
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We investigated the dynamics of canine distemper virus (CDV) in grizzly bears (Ursus arctos) and wolves (Canis lupus) of the Greater Yellowstone Ecosystem (GYE) using serological data collected from 1984 to 2014. 565 sera samples were obtained from 425 unique grizzly bears (134 females and 291 males) from 1984 to 2014 and 319 sera samples were obtained from 285 unique wolves (130 females and 155 males) from 1996 to 2014. Here we provide the unique identifier for each individual, the species and sex, the date the individual was captured, the estimated birth year and age, and the CDV antibody titer results based upon serum neutralization assays.
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Acanthurid surgeonfishes are an abundant and diverse group of herbivorous fishes on coral reefs. While their contribution to trophic linkages and dynamics in coral reef systems has received considerable attention, the role of linkages involving their parasites has not. As both consumers of fish tissue and prey to microcarnivores, external parasites can play a significant role in trophic transfer between the primarily algae-based food source and the broader coral reef community. Stable isotope analysis is a common tool for studying trophic linkages, which can be used for studies involving parasites. These data were collected to examine the stable isotope ecology (13C and 15N) of copepod (Caligus atromaculatus) and...
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We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
The relationships between Mycoplasma agassizii, a causative agent of upper respiratory disease (URTD), and desert tortoise (Gopherus agassizii), generally illustrate the complexities of disease dynamics in wild vertebrate populations. In this review, we summarize current understanding of URTD in Mojave desert tortoise populations, we illustrate how inadequate knowledge of tortoise immune systems may obfuscate assessment of disease, and we suggest approaches to future management of URTD in desert tortoise populations. We challenge the view that M. agassizii causes consistent levels of morbidity and/or mortality across the Mojave desert. Instead, URTD may be described more accurately as a context-dependent disease....
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The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae egg/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm2...
Influenza A viruses are one of the most significant viral groups globally with substantial impacts on human, domestic animal and wildlife health. Wild birds are the natural reservoirs for these viruses, and active surveillance within wild bird populations provides critical information about viral evolution forming the basis of risk assessments and counter measure development. Unfortunately, active surveillance programs are often resource-intensive, and thus enhancing programs for increased efficiency is paramount. Machine learning, a branch of artificial intelligence applications, provides statistical learning procedures that can be used to gain novel insights into disease surveillance systems. We use a form of...
These data represent capture mark recapture data from toads, and results of testing for Bd (Batrachochytrium dendrobatidis) the pathogen that causes amphibian chytridiomycosis on individuals. The data span from 2004-2016 at three sites in Montana where boreal toads were declining. The data also include temperature measurements at the sites and information on the egg counts and Bd prevalence from Columbia spotted frogs that are co-occurring at one of the sites.
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These data represent the standard curve values as well as measurements of cytokine and reference gene transcripts from blood samples collected from Florida manatees and quantified using quantitative PCR.
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The size and sex of each of the Burmese pythons swabbed in this study for the SFD-causing (snake fungal disease) Ophidiomyces ophiodiicola pathogen is given along with the real time PCR swab result.
This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, hatchling American kestrels (Falco sparverius) were exposed to the flame retardant isopropyl triphenyl phosphate (ITP) and then challenged with a synthetic analogue of viral double-stranded RNA, polyinosinic:polycytidylic acid (poly I:C). Control birds were challenged with vehicle only or vehicle and poly I:C. At euthanasia, spleen, thymus, and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative...
From 2003-2018, USGS researchers and collaborators conducted mark-recapture studies of the boreal toad at the Lost Trail National Wildlife Refuge in northwestern Montana, USA. The datasets included here contain information on individual toad capture history, body size, and disease status. These data were collected annually over the 16-year period at up to 11 breeding sites per year on the refuge. We include datasets for (1) male toad captures over time, (2) female toad captures over time, (3) male disease status (tested - positive or negative, or not tested in a given year), (4) all toad length and weight data over time, and (5) male toad size and body condition over time.
The National Wildlife Health Center (NWHC) is a science center of the United States Geological Survey. The NWHC was established in 1975 as a biomedical laboratory dedicated to assessing the impact of disease on wildlife and to identifying the role of various pathogens in contributing to wildlife losses.
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Data release contains two shapefiles related to poultry houses on the Delmarva Peninsula (Delaware, Eastern Shore Maryland and, Eastern Shore Virginia). One dataset is a polygon shapefile representing 6013 poultry houses identified using 2016 and 2017 aerial imagery from the U.S. Department of Agriculture's National Aerial Imagery Program (NAIP). The second dataset is a point shapefile of inactive houses which displayed similar characteristics to the active houses except were overgrown or in a state of disrepair (in some cases only the building's footprint remains).
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A total of 200 soil samples were collected in the Greater Yellowstone Ecosystem in Grand Teton National Park and the National Elk Refuge in July 2019 for chronic wasting disease (CWD) monitoring purposes. To collect samples from locations where ungulates are most likely to be shedding or encountering CWD, we targeted elk collar data locations, known migration routes of mule deer and elk, and areas where yearly elk supplemental feeding generally occurs on the National Elk Refuge. We sampled 10 transects in Grand Teton National Park and 10 transects in the National Elk Refuge, each 1,000 meters in length. We collected soil samples every 100 meters along the transect for a total of 10 samples per transect. Terra Core...
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******These data are preliminary or provisional and are subject to revision. They are being provided to meet the need for timely best science. The data have not received final approval by the U.S. Geological Survey (USGS) and are provided on the condition that neither the USGS nor the U.S. Government shall be held liable for any damages resulting from the authorized or unauthorized use of the data. Invasive species are a subset of non-native (or nonindigenous) species, and knowing what species are non-native to a region is a first step to managing invasive species. This is the second update to the dataset "First comprehensive list of non-native species established in three major regions of the United States" supporting...
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The life cycle of Leucocytozoon simondi is Illustrated in a series of color photomicrographs of the various stages of development in the blackfly vector and in the tissues and blood of Anatidae. A brief discussion of the life cycle is presented and taxonomic status of the parasite is reviewed. The bibliography includes references to the stages depicted and other phases of the development, morphology and pathogenesis of .L. simondi.
Categories: Publication; Types: Citation; Tags: Wildlife Disease
Two populations of boreal toads (Bufo boreas) experienced drastic declines in abundance in the late 1990s. Evidence supported the hypothesis of disease (the chytrid fungus, Batrachochytrium dendrobatidis) as the cause of these declines, but other hypotheses had not been evaluated. We used an 11-year capture-recapture data set to evaluate weather and disease as causes of these declines. We developed sets of mathematical models that reflected hypothesized relationships between several weather variables and annual survival rates of adult males in these populations. In addition, models that reflected the possibility that the declines were caused by an introduced fungus were developed. All models were fit to the data...
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This dataset provides the results of a national survey of the conterminous U.S. for the salamander chytrid fungus Batrachochytrium salamandrivorans (Bsal) conducted by the U.S. Geological Survey Amphibian Research and Monitoring Initiative from May 2014 to August 2017. Sites were sampled by capturing amphibians by hand or by traps or nets that were then swabbed individually using methods that prevent sample contamination. All swabs were then analyzed using a real-time TaqMan PCR for detection of B. salamandrivorans on the extracted DNA. The data consist of locality information and data on the individual sampled, as well as the result of the test for B. salamandrivorans. No cases of B. salamandrivorans were detected...


map background search result map search result map Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples Quantitative PCR standard curve and transcript values for the Florida manatee (Trichechus manatus latirostris), derived from 2013-14 manatee health assessments in Crystal River and Titusville, Florida Canine distemper virus antibody titer results for grizzly bears and wolves in the Greater Yellowstone Ecosystem 1984-2014 Data from a national survey for the amphibian chytrid fungus Batrachochytrium salamandrivorans A comprehensive list of non-native species established in three major regions of the United States: Version 3.0 (provisional release) Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles Data from Burmese pythons swabbed for the presence of SFD-causing Ophidiomyces in southwest Florida Poultry Feeding Operations on the Delaware, Maryland, and Virginia Peninsula from 2016 to 2017 Soil sample locations for chronic wasting disease prion surveillance in Grand Teton National Park and National Elk Refuge 2019 Stable isotope dynamics of herbivorous reef fishes and their ectoparasites: 2012, 2013, 2018 Mark-recapture data for a boreal toad metapopulation at the Lost Trail National Wildlife Refuge, Montana (2003-2018) Mark-recapture data for a boreal toad metapopulation at the Lost Trail National Wildlife Refuge, Montana (2003-2018) Stable isotope dynamics of herbivorous reef fishes and their ectoparasites: 2012, 2013, 2018 Quantitative PCR standard curve and transcript values for the Florida manatee (Trichechus manatus latirostris), derived from 2013-14 manatee health assessments in Crystal River and Titusville, Florida Poultry Feeding Operations on the Delaware, Maryland, and Virginia Peninsula from 2016 to 2017 Data from Burmese pythons swabbed for the presence of SFD-causing Ophidiomyces in southwest Florida Canine distemper virus antibody titer results for grizzly bears and wolves in the Greater Yellowstone Ecosystem 1984-2014 Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples Data from a national survey for the amphibian chytrid fungus Batrachochytrium salamandrivorans A comprehensive list of non-native species established in three major regions of the United States: Version 3.0 (provisional release)