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Freeze–thaw cycles can promote soil N losses as a result of microbial and root cell lysis; however, minimal freeze–thaw effects have typically been observed in studies that have imposed moderate temperature cycles. We conducted laboratory incubations on surface soil (top 3 cm) collected in a temperate old field from late fall through mid-winter to examine how variation in freeze–thaw amplitude, number, timing of collection, and freezing rate altered soil extractable N. We varied freeze–thaw amplitude by imposing minimum cycle temperatures of 0, −1, −2, −5, and −10°C for a series of either one or two cycles and held control samples constant at 3°C. We also examined the effects of freezing rates of 1, 3, and 30°C...
Freeze–thaw cycles can promote soil N losses as a result of microbial and root cell lysis; however, minimal freeze–thaw effects have typically been observed in studies that have imposed moderate temperature cycles. We conducted laboratory incubations on surface soil (top 3 cm) collected in a temperate old field from late fall through mid-winter to examine how variation in freeze–thaw amplitude, number, timing of collection, and freezing rate altered soil extractable N. We varied freeze–thaw amplitude by imposing minimum cycle temperatures of 0, −1, −2, −5, and −10°C for a series of either one or two cycles and held control samples constant at 3°C. We also examined the effects of freezing rates...