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To study the mechanisms, pathways, and rates of transformation of carbon and nitrogen compounds (natural and contaminant) mediated by microorganisms in aquatic habitats and identify factors controlling these transformations and to examine the effect that these transformations have upon other biogeochemical processes.
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Avian botulism toxicity is a common cause of death to water and shore birds that live near or migrate through Lake Michigan. The botulism neuro-toxin type E (bontE) gene is responsible for the production of botulinum neurotoxin type E. Quantitative Polymerase Chain Reaction (qPCR) was performed using a Step One Plus Thermocycler (Applied Biosystems) and protocol described in Getchell and others, 2011, Journal of Aquatic Animal Health. The assay was used to assess microbial community DNA obtained from environmental samples that were collected by Great Lakes Science Center and by National Park Service from 2011 to 2014 for the bontE gene. Samples were obtained by ponar grab or by divers and matrices collected...
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These data were collected as part of a voluntary initiative to create a White-Nose Syndrome Diagnostic Laboratory Network among laboratories participating in research and surveillance for Pseudogymonascus destructans (Pd) - the fungal pathogen causing White-Nose Syndrome in bats. Pd_qPCR_InterlaboratoryLODdata.xlsx is raw qPCR data from multiple laboratories running serial dilutions of Pd gBlock in known concentrations for the collectively used Muller (2013) Pd qPCR assay. Pd_qPCR_InterlaboratoryResults_LOD.xlsx contains the data output for each laboratory from running a generic LOD/LOQ calculator script. the generic LOD/LOQ calculator script is available at:https://github.com/cmerkes/qPCR_LOD_Calc. Pd_qPCR_InterlaboratoryPTResults_PanelData.xlsx...
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Water resources around the world are contaminated with per- and polyfluoroalkyl substances (PFAS) due to releases from point sources on military installations, fire training centers, and chemical manufacturing sites. Non-point sources have also been identified including wastewater effluent, landfills, and biosolids application. PFAS are a major concern to myriad stakeholders as some are known to bioaccumulate, they have eco-toxicity effects, and they are highly recalcitrant. PFAS are often called “forever chemicals” due to their environmental persistence but many precursor PFAS are transformed in the environment by microbes. Recent work has shown that PFAS can be biologically degraded in laboratory studies, but...
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Background Heavy metals, phosphorus, and organic contaminants in water and sediments of the lower Genesee River, resulted in the designation of fourteen beneficial uses as impaired in the Rochester Embayment Area of Concern (AOC). The benthic macroinvertebrate community or “benthos” Beneficial Use Impairment (BUI) was designated as degraded in the Genesee River because the New York State Department of Environmental Conservation (NYSDEC) impairment metrics generally identified slight to moderate impacts through the 1990s and 2000s. Accumulation of “sediments on more suitable substrates” and “contaminants in sediment of the Genesee River related to past municipal and industrial waste-water treatment plant discharges...
A number of investigations have shown that photoreduction of Fe(III) causes midday accumulations of dissolved Fe(II) in rivers and lakes, leading to large diel (24-h) fluctuations in the concentration and speciation of total dissolved iron. Less well appreciated is the importance of photoreduction in providing chemical energy for bacteria to thrive in low pH waters. Diel variations in water chemistry from the highly acidic (pH 2.3 to 3.1) Río Tinto, Río Odiel, and Río Agrio of southwestern Spain (Iberian Pyrite Belt) resulted in daytime increases in Fe(II) concentration of 15 to 66 µM at four diel sampling locations. Dissolved Fe(II) concentrations increased with solar radiation, and one of the stream sites showed...
Data are presented on the physiological diagnostics of cyanobacterial communication with higher plants in natural symbioses (plant syncyanoses) and in model associations, as well as on the interaction of the partners without spatial integration. Emphasis is placed on changes in cyanobacterial features important for symbiogenesis. The multicomponent composition and the possible nature of the factors that enable partner communications are discussed with hormogonia formation and taxis as an example. Published in Microbiology, volume 75, issue 4, on pages 465 - 469, in 2006.
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Background Past water-quality issues in the St. Lawrence River at Massena, NY resulted in a determination that selected beneficial uses were impaired in a surrounding Area of Concern (AOC) and on the Canadian side of the international boundary (Cornwall, Ontario). The benthic macroinvertebrate community or “benthos” Beneficial Use Impairment (BUI) was designated degraded because impairment metrics were unavailable or inconclusive. Recent sampling efforts by New York State Department of Environmental Conservation (NYSDEC) as part of their Rotating Integrated Basin Studies (RIBS) program indicate that macroinvertebrate communities in some sections of the St. Lawrence River and its tributaries in the Massena AOC are...
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Background Past water-quality issues in the St. Lawrence River at Massena, NY resulted in a determination that selected beneficial uses may be impaired in a surrounding Area of Concern (AOC) and on the Canadian side of the international boundary (Cornwall, Ontario). The plankton (phytoplankton zooplankton) Beneficial Use Impairment (BUI) was so designated because impairment metrics were unavailable or inconclusive. Recent investigations, however, suggest that plankton communities are relatively healthy and no longer threaten the local ecosystem. Thus, the BUI for plankton may now be outdated in all, or parts of, the St. Lawrence River in the Massena AOC. The primary goal for the Massena (and Cornwall) Remedial Action...
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Mono Lake is a hypersaline soda lake rich in dissolved inorganic arsenic with its primary production currently dominated by Picocystis str. ML. We set out to determine if this picoplankter could metabolize inorganic arsenic, and in doing so form unusual arsenolipids (e.g., methylated arsinoyl ribosides) as reported in other saline ecosystems and by halophilic algae. We cultivated Picocystis str. ML on an inorganic seawater-based medium with either low (37 µM) or high (1,000 µM) phosphate in the presence of arsenite (~0.4 mM), arsenate (~0.8 mM), or without arsenic additions.
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This dataset contains data tables of laboratory quality-control data associated with environmental samples analyzed for microbiological constituents at the Ohio Water Microbiology Laboratory of the U.S. Geological Survey (USGS). The environmental samples were collected across the United States by USGS National Projects and projects in Water Science Centers. These quality-control data can be used to assess the quality of microbiological data for the associated environmental samples.
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Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene...
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Data supporting "Common hydraulic fracturing fluid additives alter the structure and function of anaerobic microbial communities", describing iron reduction experiments, microbial community profiling, and statistical testing of data.
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This U.S. Geological Survey (USGS) data release provides a comprehensive dataset of water-quality results, physical-parameter measurements, hydrologic measurements, and site information collected to study the nature and extent of water quality along groundwater flow paths adjacent to glacial-kettle lakes on Cape Cod, Massachusetts. Water-quality samples were collected in 2003, 2005, and 2012 through 2018 in and near seven kettle lakes located on western Cape Cod, with most of the data collected in 2015-2017 from Ashumet Pond, which is located in the towns of Falmouth and Mashpee. Data were also collected at other lakes to compare the lake-specific influences of geochemistry and hydrology on the downgradient groundwater...
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Oil and gas (OG) wastewaters are commonly disposed of by underground injection and previous research showed that activities at a disposal facility in West Virginia affected stream biogeochemistry and sediment microbial communities downstream from the facility. Microorganisms can control the fate and transport of organic and inorganic components of OG wastewater highlighting the need to characterize the effects of OG wastewater components on microbial activity. We conducted a series of aerobic microcosm experiments to assess the influence of high total dissolved solids (TDS) and hydraulic fracturing fluid additives (2,2-dibromo-3-nitrilopropionamide (DBNPA), a biocide, and ethylene glycol, an anti-scaling additive),...
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This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Trenton, New Jersey. Microbial acetylene uptake in groundwater samples was established by mixing the groundwater with a defined mineral medium to supply nutrients and providing acetylene as the sole electron donor and carbon source. The structure of the microbial community in those enrichments was characterized as shown by...
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The Permian Basin, straddling New Mexico and Texas, is one of the most productive oil and gas (OG) provinces in the United States. OG production yields large volumes of wastewater that contain elevated concentrations of major ions including salts (also referred to as brines), and trace organic and inorganic constituents. These OG wastewaters pose unknown environmental health risks, particularly in the case of accidental or intentional releases. Releases of OG wastewaters have resulted in water-quality and environmental health effects at sites in West Virginia (Akob, et al., 2016, Orem et al. 2017, Kassotis et al. 2016) and in the Williston Basin region in Montana and North Dakota (Cozzarelli et al. 2017, Cozzarelli...
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This environmental dataset consists of 56 sediment, 24 Cladophora and 22 water samples collected from beaches at Glen Haven, Good Harbor, Platte Bay and Esch road beach, located within The Sleeping Bear Dunes National Lakeshore, Leelanau and Benzie counties, Michigan. The dataset includes matrix type, location, data, time and the qPCR reported value for each sample collected.


map background search result map search result map Estimating chronic toxicity of waters from the St. Lawrence River at Massena Area-of-Concern using two plankton species Sediment toxicity and status of benthic invertebrate communities in the St. Lawrence River and its tributaries within the Massena Area-of-Concern Sediment Toxicity and Condition of Benthic Invertebrate Communities in the Rochester Embayment Area-of-Concern Data for common hydraulic fracturing fluid additives alter the structure and function of anaerobic microbial communities Sleeping Bear Dunes National Lakeshore qPCR Data. Collection Year: 2012 Avian Botulism qPCR data, Leelanau Peninsula, Michigan. Years 2011-2014 Ohio-Kentucky-Indiana Water Science Center Water-Quality Data in and near Groundwater Flow-Through Kettle-Hole Lakes, Western Cape Cod, Massachusetts, 2003 - 2018 Growth Characteristics of Syntrophotalea acetylenivorans, strain SFB93, sp. nov., a diazotrophic, acetylenotrophic anaerobe isolated from San Francisco Bay intertidal sediments Laboratory quality-control data associated with samples analyzed for microbiological constituents at the USGS Ohio Water Microbiology Laboratory Growth of cultured Picocystis strain ML in the presence of arsenic, and occurrence of arsenolipids in these Picocystis as well as biota and sediment from Mono Lake, California Acetylene Consumption and Dechlorination by a Groundwater Microbial Enrichment Culture Data on the Enrichment and Isolation of the Acetylenotrophic and Diazotrophic Isolate Bradyrhizobium sp. strain I71 (ver 2.0, September 2022) Data on the Effects of Oil and Gas Wastewater Components on Microbial Community Structure and Function Geochemistry and microbiology data collected to study the effects of oil and gas wastewater dumping on arid lands in New Mexico Microbial Populations in PFHxSAm (perfluorohexane sulfonamido propyl amine) Biodegradation Microcosms Pd qPCR Interlaboratory Testing Results Data on the Enrichment and Isolation of the Acetylenotrophic and Diazotrophic Isolate Bradyrhizobium sp. strain I71 (ver 2.0, September 2022) Acetylene Consumption and Dechlorination by a Groundwater Microbial Enrichment Culture Water-Quality Data in and near Groundwater Flow-Through Kettle-Hole Lakes, Western Cape Cod, Massachusetts, 2003 - 2018 Estimating chronic toxicity of waters from the St. Lawrence River at Massena Area-of-Concern using two plankton species Sediment toxicity and status of benthic invertebrate communities in the St. Lawrence River and its tributaries within the Massena Area-of-Concern Growth of cultured Picocystis strain ML in the presence of arsenic, and occurrence of arsenolipids in these Picocystis as well as biota and sediment from Mono Lake, California Microbial Populations in PFHxSAm (perfluorohexane sulfonamido propyl amine) Biodegradation Microcosms Growth Characteristics of Syntrophotalea acetylenivorans, strain SFB93, sp. nov., a diazotrophic, acetylenotrophic anaerobe isolated from San Francisco Bay intertidal sediments Sediment Toxicity and Condition of Benthic Invertebrate Communities in the Rochester Embayment Area-of-Concern Data on the Effects of Oil and Gas Wastewater Components on Microbial Community Structure and Function Data for common hydraulic fracturing fluid additives alter the structure and function of anaerobic microbial communities Sleeping Bear Dunes National Lakeshore qPCR Data. Collection Year: 2012 Avian Botulism qPCR data, Leelanau Peninsula, Michigan. Years 2011-2014 Geochemistry and microbiology data collected to study the effects of oil and gas wastewater dumping on arid lands in New Mexico Laboratory quality-control data associated with samples analyzed for microbiological constituents at the USGS Ohio Water Microbiology Laboratory Ohio-Kentucky-Indiana Water Science Center Pd qPCR Interlaboratory Testing Results