Filters: Tags: Flow cytometry (X)10 results (111ms)
In 2015-2016, the Ohio Division of Wildlife’s undercover law enforcement purchased 1,200 grass carp (Ctenopharyngodon idella). Fish heads and eyeballs were sent overnight to U.S. Geological Survey Wetland and Aquatic Research Center for ploidy analysis. Field and laboratory standard operating procedures were established and followed. Fish lengths, fish weights, and eyeball weights were obtained from the U.S. Fish and Wildlife Service’s feral carp ploidy program for grass carp and black carp (Mylopharyngodon piceus) and the Ohio grass carp. Internal 2µm or 4µm bead standards were used in establishing nuclear sizes from Nile tilapia (Oreochromis niloticus), known diploid (n=20) and triploid (n=20) carp blood, as well...
Data consist of flow cytometry files that were generated in the analysis of white blood cells from kestrel blood. Thus, data are in standard format that allows files created by one type of acquisition hardware and software to be analyzed by any other type.
Imaging Flow Cytometry Data for Live and Preserved Phytoplankton Samples from Owasco and Seneca Lakes, Finger Lakes Region, New York, 2020
This U.S. Geological Survey (USGS) data release contains phytoplankton classification and enumeration results from near-surface samples analyzed by imaging flow cytometry and collected as part of a harmful algae bloom (HAB) monitoring study conducted in collaboration with the New York State Department of Environmental Conservation (NYSDEC). Samples were collected biweekly from monitoring platforms in Owasco and Seneca Lakes and one bloom sample site at Emerson Park Boat Launch in Owasco Lake. The platforms were deployed from June-October in 2020. This dataset includes all routine and quality assurance/quality control samples collected at the three sampling locations. Phytoplankton were identified to the lowest possible...
Laboratory analysis assessing immune response after flame retardant exposure in American kestrels, Falco sparverius, through 21 days post-hatch
Laboratory analysis of innate American kestrel, Falco sparverius, immune response after exposure to flame retardant, isopropylphenyl phosphate (ITP) through 21 days post hatch. Data consist of flow cytometry files that were generated in the analysis of white blood cells from kestrel blood. Thus, data are in standard format that allows files created by one type of acquisition hardware and software to be analyzed by any other type.
Laboratory and Field Data for a Performance Evaluation of the PhytoFind, an In-Place Phytoplankton Classification Tool
This data release presents the laboratory and field results of a performance evaluation conducted on the Turner Designs, Inc., PhytoFind, an in-place phytoplankton classification tool. The laboratory evaluation included tests that were designed to characterize PhytoFind performance under a range of conditions that may affect phytoplankton fluorescence and sensor response. Laboratory tests included a range of phytoplankton types, morphologies, and biomass; water temperatures; and matrix challenges. Laboratory evaluations on the PhytoFind were performed at U.S. Geological Survey offices in Orlando, Florida and Troy, New York on various dates from July to November 2019. Data from a moving-boat survey was designed to...
Biomarkers of laboratory-exposed Northern Leopard frogs to neonicotinoids: DNA cell cycle, fragmentation, and genome size
Data were collected on whole blood DNA parameters including cell cycle, DNA fragmentation, and genome size obtained from Northern leopard frogs exposed to neonicotinoid insecticides in the laboratory. Data consist of flow cytometry files that were generated in the analysis of the frog blood.
Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area (1999-2006)
Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among sub-basins within LMNRA. To investigate whether environmental contaminant exposure is associated with alterations in male common carp (Cyprinus carpio) gamete quality and endocrine- and reproductive parameters, data were collected among sub-basins (n = 7) over 7 years (1999 to 2006). Endpoints included the sperm quality parameters of motility, viability, mitochondrial membrane potential, count, morphology, and DNA fragmentation; plasma components were vitellogenin (VTG),...
Data were collected from Xenopus laevis tadpoles exposed to clothianidin and thiamethoxam in two concentrations each, 100 ppm and 20 ppm. Gene expression was generated from brains and livers, and liver cells provided metabolic activity data.
Ploidy and genome size estimates of artificially produced larval pallid sturgeon, Scaphirhynchus albus
Data consist of flow cytometry files (.FCS) that were generated in the analysis of larval pallid sturgeon specimens. Nuclei from produced larval sturgeon somatic cells were analyzed using flow cytometry to estimate genome size and assign ploidy. Freshly collected rainbow trout (Oncorhynchus mykiss), largemouth bass (Micropterus salmoides), or pallid sturgeon blood were used as external controls, whereby the fish blood samples were analyzed in separate tubes from the cells of prepared sturgeon larvae. All samples at 1 x 10^6 cells/mL were incubated in a propidium iodide solution to stain DNA, then analyzed by flow cytometry. Analysis software generated histograms from the experimental samples and the control standard...
Phytoplankton Community Composition and Abundance in Lake Okeechobee and the Okeechobee Waterway, Florida, USA, July and August 2019
This data release contains phytoplankton classification and enumeration results from two moving boat surveys conducted on July 15 - 18, 2019 and August 19 - 22, 2019. These data were collected from the Caloosahatchee River and estuary, the St. Lucie River and estuary, and Lake Okeechobee with funding provided by the USGS Priority Ecosystems Greater Everglades Program.