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A positive role for biodiversity is assumed for managed ecosystems. We conducted a 12-year study of this sustainability principle, through separate manipulation of management intensity and crop diversity. The site was located in southwest Michigan, representative of rain-fed production, with high climate variability and well-drained soils. Provisioning services of grain and protein yield were monitored, simultaneous with supporting services of soil fertility, C and N, and regulating services associated with water quality (N-use efficiency and nitrate-N leached in gravimetric lysimeters). Surprisingly, a strong role for management was shown, and almost nil for crop diversity. Organic management (ORG) sustained soil...