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Mono Lake is a hypersaline soda lake rich in dissolved inorganic arsenic with its primary production currently dominated by Picocystis str. ML. We set out to determine if this picoplankter could metabolize inorganic arsenic, and in doing so form unusual arsenolipids (e.g., methylated arsinoyl ribosides) as reported in other saline ecosystems and by halophilic algae. We cultivated Picocystis str. ML on an inorganic seawater-based medium with either low (37 µM) or high (1,000 µM) phosphate in the presence of arsenite (~0.4 mM), arsenate (~0.8 mM), or without arsenic additions.
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These metadata sets present the comprehensive geochemical composition of solid and water samples from the site of a 11.4ML (million liters) wastewater spill discovered in January, 2015. Analyses of a pipeline sample (analyses of select analytes), supplied by the North Dakota Department of Health are also included. The spill was near Blacktail Creek, north of Williston, ND. The leak was from a pipeline located approximately 70m from Blacktail Creek. The creek flows 17km before entering the Little Muddy River, a tributary to the Missouri River. The study included samples collected in waters upstream and downstream from Blacktail Creek in February and June 2015, June 2016, and June 2017. These data sets include field...
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The Marcellus Shale Energy and Environment Laboratory (MSEEL) site is a long-term field site and laboratory at the Northeast Natural Energy LLC (NNE) production facility, adjacent to the Monongahela River, located in western Monongalia County, West Virginia, USA. NNE began drilling two horizontal production wells, MIP (Morgantown Industrial Park) -5H and MIP-3H, in the Marcellus Shale in 2014. The wells were completed in December 2015. Large volumes of wastewater are generated with natural gas production. These wastewaters contain organic and inorganic chemical constituents from fracturing fluids used during drilling and stimulation of gas in host rocks/shale, as well as chemical compounds that are derived from...
Categories: Data; Tags: Energy Resources, Environmental Health, Geochemistry, MSEEL, Marcellus Shale Energy and Environment Laboratory, Morgantown, All tags...
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This U.S. Geological Survey (USGS) Data Release is focused on the geochemistry of wastewater (including flowback and produced water) samples, co-produced with natural gas, collected from the Marcellus Shale Energy and Environment Laboratory (MSEEL) site. MSEEL is a long-term field site and laboratory at the Northeast Natural Energy LLC (NNE) production facility, adjacent to the Monongahela River, located in western Monongalia County, West Virginia, USA. NNE began drilling two horizontal production wells, MIP (Morgantown Industrial Park) -5H and MIP-3H, in the Marcellus Shale in 2014. The wells were completed in December 2015. Large volumes of wastewater are generated with natural gas production. These wastewaters...
Categories: Data, Data Release - Revised; Tags: MSEEL, Marcellus Shale Energy and Environment Laboratory, Marcellus Shale, Morgantown, USGS Science Data Catalog (SDC), alkalinity, All tags...
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Oil and gas (OG) wastewaters are commonly disposed of by underground injection and previous research showed that activities at a disposal facility in West Virginia affected stream biogeochemistry and sediment microbial communities downstream from the facility. Microorganisms can control the fate and transport of organic and inorganic components of OG wastewater highlighting the need to characterize the effects of OG wastewater components on microbial activity. We conducted a series of aerobic microcosm experiments to assess the influence of high total dissolved solids (TDS) and hydraulic fracturing fluid additives (2,2-dibromo-3-nitrilopropionamide (DBNPA), a biocide, and ethylene glycol, an anti-scaling additive),...
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The Marcellus Shale Energy and Environmental Laboratory (MSEEL) field site in Morgantown, WV was established by West Virginia University in order to increase understanding of factors that affect resource recovery and environmental impacts from unconventional oil and gas development. The site, which is located in the Morgantown Industrial Park (MIP) adjacent to the Monongahela River, includes one well pad which hosts four horizontal gas wells that target the Marcellus Shale approximately 7400 ft below ground level. As part of the MSEEL study, the MIP 5H well was drilled in 2015. Water samples were collected from the holding pond used to hydraulically fracture the MIP 5H well (API 47-061-01699), and a time series...
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This U.S. Geological Survey (USGS) Data Release is focused on the geochemistry of wells within the oil zone and groundwater monitoring wells away from the oiled zone at the National Crude Oil Spill Fate and Natural Attenuation Research Site, Bemidji MN (USA) from 1985-2015. The site located in Beltrami County is where a high-pressure pipeline carrying crude oil burst in 1979 and spilled approximately 1.7 million liters (10,700 barrels) of crude oil into glacial outwash deposits. Researchers and scientists from government agencies, academic institutions, the regulatory community, and private companies have conducted extensive investigations of groundwater geochemistry in hopes of understanding the evolution of plumes...
Tags: BTEX, Beltrami, Bemidji, Environmental Health, Geochemistry, All tags...
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The U.S. Geological Survey (USGS) collected porewater samples from nine suction lysimeters in 2018, 2019, and 2021 for analysis of organic and inorganic constituents from the National Crude Oil Spill Fate and Natural Attenuation Research Site near Bemidji, MN. In August of 1979, approximately 1,700,000 L (liters), or 10,700 barrels, of crude oil spilled onto a glacial outwash aquifer. Sampled lysimeters included L310-1.5, L310-4.5, L1802-1.8, L9014-1.5, L9014-3.0, L9014-4.5, L9017-1.3, L9017-2.5, and L9017-3.7. This data release presents data on analytes that are important indicators of biodegradation processes. Some of these analytes, if present in elevated concentrations, can be a concern regarding potential effects...
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This USGS data release includes all the data presented in peer-reviewed publication entitled "Degradation of MCHM (4-methylcyclohexanemethanol) in Sediments from Elk River, West Virginia". We conducted experiments on crude MCHM to examine photooxidation or biodegradation. We also assessed the potential of sediments to serve as a long-term source of MCHM and well as the potential for native microbial communities to catalyze the anaerobic breakdown of MCHM. We developed a quantitative method to measure the 2 isomers (cis- and trans-) of 4-MCHM, using solid phase micro-extraction (SPME), which had a minimum detection limit of 5 µg/L. The data release shows that the abiotic degradation experiments showed no evidence...


    map background search result map search result map MCHM Degradation Data Release Aqueous and solid phases partitioning of elemental constituents associated with Marcellus Shale Energy and Environment Laboratory (MSEEL) gas well produced wastewater, Morgantown, WV, 2016 - 2019 Growth of cultured Picocystis strain ML in the presence of arsenic, and occurrence of arsenolipids in these Picocystis as well as biota and sediment from Mono Lake, California Geochemistry Data from Samples Collected in 2015-2017 to study an OG wastewater spill in Blacktail Creek, North Dakota Data on the Effects of Oil and Gas Wastewater Components on Microbial Community Structure and Function Geochemistry Data for Wastewater Samples Collected at a Separator Tank and from an On-Site Storage Tank at the Marcellus Shale Energy and Environment Laboratory (MSEEL) 2015-2019, Morgantown Industrial Park (MIP), West Virginia (ver. 2.0, May 2023) Geochemistry data collected (1985-2015) for understanding the evolution of groundwater-contaminant plume chemistry emanating from legacy contaminant sources, an example from a long-term crude oil spill near Bemidji, Minnesota Analyses of Select Organic and Inorganic Data Collected from Lysimeters Installed at the Bemidji Crude Oil Spill Site, Minnesota, 2018, 2019, 2021 Organic Compounds Identified via Gas Chromatography-Mass Spectrometry in Produced Water Samples Collected at the Marcellus Shale Energy and Environment Laboratory (MSEEL) 2015-2019, Morgantown Industrial Park (MIP), West Virginia Groundwater and soil gas data, methods, and quality assurance information for samples collected to determine ancient carbon distributions at Red Hill, Bulk Fuel Storage Facility, O'ahu, Hawai'i 2022-2023 (COPY) Aqueous and solid phases partitioning of elemental constituents associated with Marcellus Shale Energy and Environment Laboratory (MSEEL) gas well produced wastewater, Morgantown, WV, 2016 - 2019 Organic Compounds Identified via Gas Chromatography-Mass Spectrometry in Produced Water Samples Collected at the Marcellus Shale Energy and Environment Laboratory (MSEEL) 2015-2019, Morgantown Industrial Park (MIP), West Virginia Geochemistry Data for Wastewater Samples Collected at a Separator Tank and from an On-Site Storage Tank at the Marcellus Shale Energy and Environment Laboratory (MSEEL) 2015-2019, Morgantown Industrial Park (MIP), West Virginia (ver. 2.0, May 2023) Analyses of Select Organic and Inorganic Data Collected from Lysimeters Installed at the Bemidji Crude Oil Spill Site, Minnesota, 2018, 2019, 2021 Groundwater and soil gas data, methods, and quality assurance information for samples collected to determine ancient carbon distributions at Red Hill, Bulk Fuel Storage Facility, O'ahu, Hawai'i 2022-2023 (COPY) Geochemistry data collected (1985-2015) for understanding the evolution of groundwater-contaminant plume chemistry emanating from legacy contaminant sources, an example from a long-term crude oil spill near Bemidji, Minnesota Data on the Effects of Oil and Gas Wastewater Components on Microbial Community Structure and Function MCHM Degradation Data Release