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Acetylene fermentation assays, nitrogen fixation assays, and growth studies were performed with Pelobacter sp. strain SFB93 and Pelobacter acetylenicus DSM3246. Data includes concentrations of acetylene and ethylene over time, and growth measured with OD680 and cell counts.
The data document the results of several microbe bioassays performed by the USGS on Phragmites australis plants, including those performed on mature leaves, seedlings, and dead leaf tissues exploration of the literature to find accounts of microbes associated with Phragmites worldwide. For the bioassays, we prepared 162 pure cultures isolated from Phragmites plants in North America along the east coast, Florida, the Gulf of Mexico, and the Great Lakes area, 125 of which were from a previous study, and 38 represent new collections. The DNA sequences used to identify the 37 new collections are included. Microbes were isolated from plants collected from 2015-2018. We performed assays using both North American plant...
Site-specific multiple linear regression models were developed for one beach in Ohio (three discrete sampling sites) and one beach in Pennsylvania to estimate concentrations of Escherichia coli (E. coli) or the probability of exceeding the bathing-water standard for E. coli in recreational waters used by the public. Traditional culture-based methods are commonly used to estimate concentrations of fecal indicator bacteria, such as E. coli; however, results are obtained 18 to 24 hours post sampling and do not accurately reflect current water-quality conditions. Beach-specific mathematical models use environmental and water-quality variables that are easily and quickly measured as surrogates to estimate concentrations...
Three regression models were developed for describing variability of host-specific flux data in eight Great Lakes tributaries. Models include one for Lachnospiraceae flux, one for human bacteroides flux, and one for ruminant bacteroides flux. Models were developed using the R project for statistical computing with core functionality and the survival, smwrBase, and smwrQW packages. Predictor variables for these models are included in the data file and input files provided. These include sampling dates and times, rainfall depth, percent of watershed underlain by tile drainage, cattle population (for the ruminant marker model) and human population (for the human marker models). Sample collection and laboratory analyses...
Categories: Data;
Tags: Bacteria,
Great Lakes Region,
Regression analysis,
Surface water quality,
USGS Science Data Catalog (SDC)
Controlled waterborne exposures of Pacific herring (Clupea pallasii) to Vibrio anguillarum and V. ordalii failed to result in overt signs of disease or mortality from vibriosis. Cumulative mortalities among Vibrio - exposed Pacific herring (3.3 - 5.0%) were similar to those of saline-exposed negative controls (10%) and significantly less (P < 0.001) than those of Vibrio - exposed Chinook salmon (60 - 97%), a known susceptible species. Gross signs of disease did not occur on any dead or surviving Pacific herring; however, exposed Chinook salmon demonstrated classic gross signs of vibriosis. The results indicate that early reports of presumed vibriosis in Pacific herring during the 1950’s were likely misdiagnosed...
Categories: Data;
Tags: Ecology,
USGS Science Data Catalog (SDC),
Wildlife Disease,
bacteria,
biota,
The diagnosis of bacterial disease in freshwater unionid mussels has been hindered by a lack of baseline information regarding the microbial communities associated with these animals. In this study, we cultured and identified bacteria from the hemolymph of stable mussel populations from the upper Mississippi River basin and compared results to mussel populations associated with a mortality event in the Clinch River, VA and TN. Several bacterial genera were consistently identified across mussel species and locations, appearing to be part of the natural bacterial flora. One noteworthy isolate was identified from the Clinch River. Yokenella regensbergei was found with relatively high prevalence during the mortality...
Categories: Data;
Tags: Bacteria,
Clinch River, Virginia, USA,
Clinch River,Tennessee, USA,
Hemolymph,
USGS Science Data Catalog (SDC),
Water-quality data were collected by the Providence Water Supply Board (PWSB) from tributaries in the Scituate Reservoir drainage area, October 1, 2017 through September 30, 2022 (water years 2018 through 2022). Water-quality samples were collected by the PWSB either monthly or quarterly at fixed stations on 38 tributaries in the Scituate Reservoir drainage area, Rhode Island. These data were used to calculate instantaneous loads and yields of constituents in reports by the U.S. Geological Survey. Water-quality samples were collected following a strict sampling schedule so that water-quality samples would be representative of various weather conditions. Samples were analyzed at the PWSB water-quality laboratory...
These data show grass crop and model species response to toxic chemicals (Arsenic (As)) and humic acids. Experiments were performed by collaboration between the U.S. Geological Survey, Rutgers University, and Rey Juan Carlos University. A series of individual experiments investigated beneficial effects of endophytic bacteria on grass crop growth and resilience to known plant toxicity.
Categories: Data;
Tags: Ecology,
Genetics,
North America,
USGS Science Data Catalog (SDC),
bacteria,
Data supporting the establishment of Syntrophotalea acetylenivorans SFB93 as a new microbial species. Data release includes growth curve and preferred growth conditions for the species.
Categories: Data;
Tags: Aquatic Biology,
Environmental Health,
USGS Science Data Catalog (SDC),
Water Quality,
Water Resources,
To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted...
The dataset includes Escherichia coli concentrations in water and sediment collected by James Fluke in the Middle Rio Grande in Albuquerque, NM, from 2017 to 2018.
Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene...
Chemical and microbiological results, quality assurance and quality control, site location, and method information for surface water, bed sediment, and wastewater effluent collected from 34 stream locations across Iowa (United States). Environmental samples were analyzed for a suite of 29 antibiotics, plated on selective media for 15 types of bacteria growth, and DNA was extracted from culture growth and used in downstream polymerase chain reaction (PCR) assays for the detection of 24 antibiotic resistance genes. Environmental field data were collected in-situ for water temperature (°C), specific conductivity (µs/cm), dissolved oxygen (mg/L), pH (standard units), and turbidity (FNU) using a multiparameter sonde...
Categories: Data;
Tags: Environmental Health,
Iowa,
USGS Science Data Catalog (SDC),
United States,
Water Quality,
This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Trenton, New Jersey. Microbial acetylene uptake in groundwater samples was established by mixing the groundwater with a defined mineral medium to supply nutrients and providing acetylene as the sole electron donor and carbon source. The structure of the microbial community in those enrichments was characterized as shown by...
Categories: Data;
Tags: Environmental Health,
Trenton West,
USGS Science Data Catalog (SDC),
Water Resources,
aquatic biology,
Oil and gas (OG) wastewaters are commonly disposed of by underground injection and previous research showed that activities at a disposal facility in West Virginia affected stream biogeochemistry and sediment microbial communities downstream from the facility. Microorganisms can control the fate and transport of organic and inorganic components of OG wastewater highlighting the need to characterize the effects of OG wastewater components on microbial activity. We conducted a series of aerobic microcosm experiments to assess the influence of high total dissolved solids (TDS) and hydraulic fracturing fluid additives (2,2-dibromo-3-nitrilopropionamide (DBNPA), a biocide, and ethylene glycol, an anti-scaling additive),...
Categories: Data;
Tags: Biochemistry,
DNA sequencing,
Energy Resources,
Environmental Health,
Fayette County,
This product consists of multiple tabular datasets and associated metadata for clinical status, gene transcripts, and lab results related to presence of Mycoplasma spp. in desert reptiles. To better understand immune responses to pathogenic infections, we conducted an experiment to quantify innate and induced immune responses using gene transcript profiles and measured induced antibody levels for Mycoplasma spp. in Mojave Desert tortoises (Gopherus agassizii). Data consists of: - Clinical Status Data - Clinical status of presence or absence of nasal discharge, eroded nares, or labored respiration in 15 captive tortoises classified as naive, exposed, or infected. - Gene Transcript Data - Gene transcription values...
The dataset includes Escherichia coli concentrations collected by the Bosque Ecosystem Monitoring Program in the Middle Rio Grande in Albuquerque, NM, from 2017 to 2020.
This study evaluated the use of medicated feeds to treat furunculosis infections caused by the bacterium Aeromonas salmonicida in lake herring (Coregonus artedi, Lesueur, 1818). The objectives of this two-phase study were to (1) determine if Coregonus artedi could be adequately and consistently infected with A. salmonicida by the methods of bath immersion or intraperitoneal (IP) injection, and (2) determine if the medicated feeds Oxytetracycline Dihydrate (Terramycin® 200 For Fish) and Florfenicol (Aquaflor®) could be used for the control of furunculosis caused by A. salmonicida. These datasets include water quality, mortality, feed weights and behavior, and qPCR data collected during both phases of the study.
The dataset contains Escherichia coli (E. coli) concentrations and loads that were measured and calculated from samples collected from the Little Blue River and its tributaries in Independence, MO. The data was collected during the 2010-2016 recreation seasons. The U.S. Geological Survey, in coordination with the City of Independence, collected the data for use in regression models to evaluation E. coli loads in the Little Blue River and its tributaries within the boundaries of the City of Independence. Further information on the methods and the results of the data collection and regression models can be found in the conference proceedings paper cited below. Citation: Flickinger, A.; Christensen, E. Evaluation...
Categories: Data;
Types: Citation;
Tags: East Fork Little Blue River,
Independence, Missouri,
Little Blue River,
bacteria,
water-quality data
Water-quality data were collected by the Providence Water Supply Board (PWSB) from tributaries in the Scituate Reservoir drainage area, October 2016 through September 2017 (water year 2017). Water-quality samples were collected by the PWSB either monthly or quarterly at fixed stations on 36 tributaries in the Scituate Reservoir drainage area. These data were used to calculate instantaneous loads and yields of constituents in a report by the U.S. Geological Survey. Water-quality samples were collected following a strict sampling schedule so that water-quality samples would be representative of various weather conditions. Samples were analyzed at the PWSB water-quality laboratory at the P.J. Holton Water Purification...
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