The 2018 lower East Rift Zone eruption and accompanying summit collapse of Kīlauea Volcano, Hawaiʻi, comprised one of the most impactful events on the volcano in the past 200 years, with hundreds of homes destroyed and major changes in the topography of the summit caldera. The opening stages of this eruptive sequence started on 30 April, when a magmatic dike began moving east from Puʻuʻōʻō, a cone with a central crater that was the vent region for Kīlauea’s 35-year middle East Rift Zone eruption starting in 1983. The rapid migration of magma from beneath Puʻuʻōʻō caused its crater floor to drop over 300 m. This data release includes a three-dimensional model of Puʻuʻōʻō and the collapse crater, constructed...
Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
Simple sequence repeats (SSRs) of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019
This dataset contains simple sequence repeat genotypes from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA.
Molecular detection of Tetracapsuloides bryosalmonae DNA from fish tissues and environmental DNA water samples collected throughout the Inter-mountain West, USA from 2011 to 2019
This dataset contains molecular diagnostic results of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue collected from the inter-mountain West, USA.
Real-time PCR results of a round robin evaluation of 5 assays that target dreissenid mussel DNA. Water samples collected from waters with and without dreissenid mussels were analyzed using these five assays in four USGS laboratories. Samples from waters without dreissenid mussels were spiked with known amounts of dreissend DNA.
T. bryosalmonae detection in fish and water, DNA sequence, and simple sequence repeat data collected in the Inter-Mountain West from 2011 to 2019
This data release contains molecular diagnostic results and molecular detection of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue and environmental DNA samples collected from the inter-mountain West, USA.
This data shows the quantification cycle at which fluorescence signals crossed a threshold fluorescence for samples analyzed as part of controlled experiments to determine whether contaminating DNA is present in samples under varying experimental conditions.
Cytochrome oxidase subunit I (COI) and internally transcribed spacer region I (ITS1) DNA sequences of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019
This dataset contains DNA sequences of two genomic regions, cytochrome oxidase subunit one and internally transcribed spacer region one, obtained from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA.