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Filters: partyWithName: National Wildlife Health Center (X) > partyWithName: Tonie E Rocke (X)

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These data were collected as part of a field trial to test the effectiveness of a sylvatic plague vaccine (see Rocke et al., 2017 for details). Vaccine and control plots were selected randomly from the available pairs at each location. Baits containing Rhodamine B, a biomarker, were distributed at each plot. At least 1 week and no more than 2 months post-baiting each year, local collaborators captured, marked, and sampled prairie dogs. Hair and whisker samples were collected from up to 50 unique prairie dogs from each plot each year. Sex, age, weight, and the identity of all current-year and prior-year recaptures were recorded for each captured animal. In the laboratory, hair/whiskers were assessed for the presence...
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In 2013, a large blinded, paired placebo-controlled field trial for the prairie dog oral sylvatic plague vaccine started in the Western US. On 17 paired plots, vaccine and placebo plots, small rodents were trapped annually for 3-5 consecutive nights (when weather allowed). Up on capture, we documented the trap numbers and the processed animals. We noted standard biological information (species, sex, age) and took samples (fleas, blood, hair and whiskers). When logistics allowed we also trapped diurnal animals. Hair and whisker samples were taped to a transparent sheet and scored for the presence (high dose 1, low dose 2) or absence (0) of Rhodamine B fluorescence (RB dataset)
In 2013, a large blinded, paired placebo-controlled field trial for the prairie dog oral sylvatic plague vaccine started in the Western US. On 17 paired plots, vaccine and placebo plots, small rodents were trapped annually for 3-5 consecutive nights (when weather allowed) and high elevation Utah plots where plague was active were more frequently trapped in 2014 and 2015. In the dataset the prevalence of flea infestation was recorded for the first annual summer sampling, it was summarized for all small rodent species caught and deer mice (Peromyscus maniculatus). Infestations were either for all flea species collected or for Aetheca wagneri only (our most abundant flea species). We used this data to assess if plague...
These data were collected as part of a field trial to test the efficacy of a sylvatic plague vaccine (see Rocke et al., 2017 for details). Vaccine and control plots were selected randomly from the available sites at each location. At least 1 week and no more than 2 months post-baiting each year, local collaborators captured, marked and sampled prairie dogs for a minimum of 3 trap days. Both plots in a pair were trapped on the same day, and trap effort (number of traps and trap days) between plots of the same pair was similar with few exceptions. Fleas were collected from up to 50 unique prairie dogs from each plot each year. Sex, age, weight, and foot length were recorded for each captured animal. In the laboratory,...
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Monkeypox virus (MPXV) is a zoonotic disease endemic in Central and West Africa and is the most virulent orthopoxvirus affecting humans since the eradication of variola virus (VARV). In order to assess their reservoir potential, wild-caught rope squirrels were experimentally infected via intranasal and intradermal exposure with a recombinant MPXV strain from Central Africa engineered to express the luciferase gene. After infection, we monitored viral replication via in vivo bioluminescent imaging and viral shedding via culture and PCR. MPXV infection in African rope squirrels caused mortality and moderate to severe morbidity, with clinical signs including pox lesions in the skin, eyes, mouth and nose, dyspnea, and...
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The data are a list of the number and species of fleas that were collected from black-tailed prairie dog burrows in Montana, North Dakota, South Dakota, Colorado, and New Mexico. Fleas were collected by swabbing ~100 burrows at 3 sites at each of 6 sites. Burrows were sampled twice each summer during 2016 and 2017.
These data were collected as part of a field trial to test the efficacy of a sylvatic plague vaccine. Treatment and control sites were selected randomly from the available sites at each location. Site pairs were a minimum of 20 acres, (with a few exceptions). Prairie dog trapping took place a minimum of two weeks post-baiting and trapping procedures were approved by the NWHC Animal Care and Use Committee as well as individual states as required.


    map background search result map search result map Monkeypox challenge of rope squirrels: data SPV field trials bait uptake data Impact of the oral Sylvatic Plague Vaccine on Non-target Small Rodents: Data Sylvatic plague vaccine field trials flea data Fleas collected from black-tailed prairie dog burrows in 2016 and 2017 Monkeypox challenge of rope squirrels: data SPV field trials bait uptake data Impact of the oral Sylvatic Plague Vaccine on Non-target Small Rodents: Data Fleas collected from black-tailed prairie dog burrows in 2016 and 2017 Sylvatic plague vaccine field trials flea data