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These data were collected as part of a field trial to test the effectiveness of a sylvatic plague vaccine (see Rocke et al., 2017 for details). Vaccine and control plots were selected randomly from the available pairs at each location. Baits containing Rhodamine B, a biomarker, were distributed at each plot. At least 1 week and no more than 2 months post-baiting each year, local collaborators captured, marked, and sampled prairie dogs. Hair and whisker samples were collected from up to 50 unique prairie dogs from each plot each year. Sex, age, weight, and the identity of all current-year and prior-year recaptures were recorded for each captured animal. In the laboratory, hair/whiskers were assessed for the presence...
The data set contains the results of experimental challenge of captive zebra finches with an American crow isolate of West Nile virus (WNV). Data include infectivity, mortality, viremia, oral shedding of virus, and serology for anti- WNV antibodies. Australian and Timor zebra finches were used in this study and both are useful as a laboratory model of an avian species with moderate susceptibility to WNV.
This dataset consists of measurements of the digestive gland in the freshwater snail Lymnaea stagnalis orally exposed to uranium. The objective of the study was to assess the morphological effects of uranium on the snail digestive gland by light microscopy. Laboratory-cultured L stagnalis were exposed to either synthetic or natural solid phases of uranium and a subset (6 controls and 6 exposed) were euthanized and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). The size of each sample, the length and width of digestive gland tubules (5 random glands per sample) and height of digestive cells...
(i) This dataset presents macroscopic and microscopic pathological findings and bacteriology of 178 tadpoles diagnosed with Severe Perkinsea Infections. (ii) Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (iii) Necropsies and gross evaluation of carcasses were carried out under a dissecting microscope. (iv) Severe Perkinsea Infection was confirmed in each specimen by histological observation of pathological changes in tissues with presence of Perkinsea-like organisms in at least one organ, including brain, eyes, gastrointestinal tract, gills, heart, liver, lungs, kidney,...
The data set contains results for nine serum biochemistries in molting Pacific black brant (Branta bernicla nigricans). These data were used to calculate reference intervals (sometimes referred to as normal values) for the nine serum biochemistries. All brant were after-hatch year. All samples were collected in 2006 and 2007 in the Teshekpuk Lake Special Area, Alaska.
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These data sets are is a compilation of bird and environmental samples obtained from 6 sites in Maricopa County, Arizona on the dates shown. Sites were only visited and sampled if they had Rosy-cheeked lovebirds coming to bird feeders at the location and with the permission of the property owner. Two swab samples were obtained from each captured bird and 3 swab samples were collected from the environment at each site. Each sample was tested by PCR for Chlamydia psittaci, Psittacine Circovirus genotype 1 [PCV-1]), and Psittacine Circovirus genotype 2 (PCV-2) and, for appropriate samples (love birds and environmental samples) and where enough sample material remained for PBFD virus Pathotype 2.
This dataset includes skin temperatures of twelve Tadarida brasiliensis held in environmental chambers maintained at 7.7 (SD 0.9) °C and 91.8 (SD 0.8) % relative humidity to induce and support hibernation for up to 3 months. Bats were randomly assigned to infected and control groups at the start of the experiment and infected with conidia of Pseudogymnoascus destructans or a sham treatment (negative control). An iBBat temperature logger (Alpha Mach, Ste-Julie, Quebec, Canada) was attached to the dorsal surface of each bat and programmed to record skin temperature every 15 minutes until the end of the experiment when the bat died or was euthanized.
Fibropapillomatosis (FP) is a tumor disease of marine turtles associated with Chelonid herpesvirus 5 (ChHV5) that has historically been refractory to growth in tissue culture. Here, we show for the first time de novo formation of ChHV5-positive intranuclear inclusions in cultured green turtle cells, which is indicative for active lytic replication of the virus. The minimal requirements to achieve lytic replication in cultured cells included 1) either in-vitro culturing of ChHV5-positive tumor biopsies or organotypic cultures consisting of ChHV5-positive turtle fibroblasts in collagen rafts seeded with ChHV5-negative or -positive turtle keratinocytes and 2) keratinocyte maturation, which occurred naturally in explanted...
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The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae egg/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm2...
Influenza A viruses are one of the most significant viral groups globally with substantial impacts on human, domestic animal and wildlife health. Wild birds are the natural reservoirs for these viruses, and active surveillance within wild bird populations provides critical information about viral evolution forming the basis of risk assessments and counter measure development. Unfortunately, active surveillance programs are often resource-intensive, and thus enhancing programs for increased efficiency is paramount. Machine learning, a branch of artificial intelligence applications, provides statistical learning procedures that can be used to gain novel insights into disease surveillance systems. We use a form of...
These data represent capture mark recapture data from toads, and results of testing for Bd (Batrachochytrium dendrobatidis) the pathogen that causes amphibian chytridiomycosis on individuals. The data span from 2004-2016 at three sites in Montana where boreal toads were declining. The data also include temperature measurements at the sites and information on the egg counts and Bd prevalence from Columbia spotted frogs that are co-occurring at one of the sites.
This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, hatchling American kestrels (Falco sparverius) were exposed to the flame retardant isopropyl triphenyl phosphate (ITP) and then challenged with a synthetic analogue of viral double-stranded RNA, polyinosinic:polycytidylic acid (poly I:C). Control birds were challenged with vehicle only or vehicle and poly I:C. At euthanasia, spleen, thymus, and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative...
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In 2007, a phase shift from corals to corallimorpharians (CM) was documented at Palmyra Atoll, Line Islands, centered around a shipwreck. Subsequent surveys revealed CM to be overgrowing the reef benthos, including corals and coralline algae, potentially placing coral ecosystems in the atoll at risk. This prompted the U.S. Fish and Wildlife Service, the lead management agency of the atoll, to remove the shipwreck. Subsequent surveys showed reversal of spread of CM around the ship impact site. We explain patterns of spread of the CM in terms of life history and local currents and show with a pilot study that pulverized bleach may be an effective tool to eradicate CM on a local scale. If applied strategically, particularly...
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In 2013, a large blinded, paired placebo-controlled field trial for the prairie dog oral sylvatic plague vaccine started in the Western US. On 17 paired plots, vaccine and placebo plots, small rodents were trapped annually for 3-5 consecutive nights (when weather allowed). Up on capture, we documented the trap numbers and the processed animals. We noted standard biological information (species, sex, age) and took samples (fleas, blood, hair and whiskers). When logistics allowed we also trapped diurnal animals. Hair and whisker samples were taped to a transparent sheet and scored for the presence (high dose 1, low dose 2) or absence (0) of Rhodamine B fluorescence (RB dataset)
This dataset includes data used to summarize trends and identify best-fit models to explain patterns in presence-absence and abundance of Pseudogymnoascus destructans (Pd) in environmental substrates and on bats within six bat hibernacula at different stages of white-nose syndrome (WNS). Data relating to environmental substrates include: dates and relative spatial locations of samples collected within study hibernacula, presence and quantity of Pd in samples based on qPCR analysis, and daily temperature parameters at each sample location on the days samples were collected. Data relating to bats include: dates and relative spatial locations of hibernating bats that were sampled, species, sex, weight(g), forearm length(mm),...
The data set contains paired-end, 100 nucleotide long RNA sequencing reads for each sample. Raw sequencing reads ranged from 18-30million reads per sample. Quality trimmed reads were mapped to the Zebra Finch reference genome with an average of 79.0-80.8% mapping rate, corresponding to 18,618 Ensembl gene IDs. Of these, 14,114 genes averaged at least 5 mapped reads across all samples and were utilized for differential expression (DE) analyses. DE analyzed two ways: as pairwise comparisons between treatments to identify specific genes with DEseq2 and as a time course grouping genes into expression paths with EBSeqHMM.
These data represent characteristics of trees used by Northern long-eared bats as roosts at three sites in Wisconsin, as well as characteristics of surrounding trees to compare characteristics of used versus available trees.
These data represent capture mark recapture data along with associated disease status for boreal toads (Anaxyrus boreas) from Wyoming and Montana from 2004-2016 and four frog species (Rana draytonii, R. muscosa, R. pretiosa, R. sierrae) from 2001-2016.
This dataset describes histopathological changes in liver, kidney, heart, skeletal muscle and intestine of captive American kestrels exposed to the second-generation anticoagulant rodenticide brodifacoum (BROD). The goal of the study was to determine the toxic range of brodifacoum by feeding birds a diet containing 0.3, 1.0, or 3.0 ug BROD/g wet weight. Birds were necropsied and examined grossly for hemorrhages or anemia, and liver, kidney, heart, pectoral muscle, and intestine was collected for histopathological evaluation. Tissues were scanned at least 100x magnification and all lesions, including hemorrhage, inflammation, and degenerative changes, were described and assigned a morphologic diagnosis with severity,...
During 2002−2015 we examined the causes of mortality in beachcast northern sea otters (Enhydra lutris kenyoni) collected from the coasts of Washington and Oregon states. A total of 333 moribund or dead sea otters were reported through opportunistic observations and 93 otter carcasses were collected for necropsy. Retrieved carcasses received a full diagnostic evaluation to determine cause of death when carcass condition permitted. Otter stranding reports were received from the entire Washington coast and northern Oregon coast, but carcasses located near areas populated by humans were most often in suitable condition for cause of death determination. Data in this release include basic demographic information on the...


map background search result map search result map Serum biochemistries of Pacific black brant SPV field trials bait uptake data Mortality trends in northern sea otters (Enhydra lutris kenyoni) collected from the coast of Washington and Oregon during 2002—2015: Data Chelonid herpesvirus 5 virus isolation data Corallimorph Invasion Palmyra: Data Occurrence of Chlamydia sp in apparently healthy birds associated with a mortality event caused by chlamydiosis: Data Impact of the oral Sylvatic Plague Vaccine on Non-target Small Rodents: Data Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles Corallimorph Invasion Palmyra: Data Occurrence of Chlamydia sp in apparently healthy birds associated with a mortality event caused by chlamydiosis: Data Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles Chelonid herpesvirus 5 virus isolation data Mortality trends in northern sea otters (Enhydra lutris kenyoni) collected from the coast of Washington and Oregon during 2002—2015: Data Serum biochemistries of Pacific black brant SPV field trials bait uptake data Impact of the oral Sylvatic Plague Vaccine on Non-target Small Rodents: Data Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data