Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environment. Understanding the environmental factors that influence the decay of eDNA and how these factors impact detection probabilities over time and space could have significant implications for eDNA survey design and data interpretation. Here we experimentally explore eDNA decay in waste materials and reproductive cells obtained...
Results from radiochemical analyses of small rodent whole bodies collected from breccia pipe uranium mines and reference locations in the Grand Canyon watershed.
These data are comprised of measurements of gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th, Ra-226) in small rodent bodies. Samples were collected at non-mining and U breccia pipe mining sites in various production stages, in the Grand Canyon watershed.
Survival and hydrodynamic behavior of grass carp eggs and larvae in relation to turbulence and in-stream obstructions
Data collected from a comprehensive set of hydrodynamic experiments conducted with live grass carp eggs and larvae, to evaluate drifting and swimming patterns with 3 different in-stream obstructions: (1) a gravel bump, (2) a single cylinder, and (3) submerged vegetation. The hydrodynamic behavior of eggs and larvae with each obstruction was continuously monitored for about 85 consecutive hours. Transient spatial distributions of the locations of eggs and larvae across the water column were generated for each flow scenario.
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.), major anions (chloride, nitrite+nitrate as nitrogen, sulfate, etc.), dissolved organic carbon, and general water quality characteristics in Canyon Mine containment pond water samples collected in calendar year 2019.
This data set includes a point cloud, digital surface model (DSM), and orthoimagery of the Fighter Strips at Palmyra Atoll, USA. Products were generated from aerial images collected in October 2016 using Unoccupied Aerial Systems (UAS, or "drones") with a Ricoh GRII digital camera. Imagery was processed using structure-from-motion photogrammetry that generates a three dimensional point cloud (.las) by identifying pixels from multiple images representing the same object and calculating the x, y, and z coordinates of that object/pixel. Point cloud data were processed to create a 6.3 cm resolution DSM (.tif) of the uppermost reflective surface (i.e., data have not been filtered for vegetation classes or height). Finally,...
Within large-river ecosystems, floodplains serve a variety of important ecological functions. A recent survey of 80 managers of floodplain conservation lands along the Upper and Middle Mississippi and Lower Missouri Rivers in the central United States found that the most critical information needed to improve floodplain management centered on metrics for characterizing depth, extent, frequency, duration, and timing of inundation. These metrics can be delivered to managers efficiently through cloud-based interactive maps. To calculate these metrics, we interpolated an existing one-dimensional HEC-RAS hydraulic model for the Middle Mississippi River, which simulated water surface elevations at cross sections spaced...
Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of longnose darter DNA. Samples were collected from sites on the St. Francis river, Missouri in 2018.
Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus
Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.
Text files containing data regarding attributes of lake trout egg thiamine concentrations from sites in lakes Huron and Michigan collected during 2019-20, sampling site locations and quality assurance quality control values for comparison to previous years (2017-18) lake trout egg thiamine concentrations.
Water quality, habitat, sampling methods and characteristics for grass carp in Truman Reservoir Missouri, 2017-2019
Diploid and triploid Grass Carp (Ctenopharyngodon Idella) tagged with acoustic transmitters were observed in Truman Reservoir, Missouri from February 2017 to March 2019 to determine habitat usage in winter. Habitat parameters were measured at used and available sites to model habitat preference. In addition, tagged fish were targeted during removal attempts to determine the efficacy of using tagged Judas Grass Carp in removing wild conspecifics. This dataset includes information on tagged Grass Carp and habitats they selected as well as capture events and characteristics of the wild fish caught during these attempts.
Water surface elevation longitudinal profile data for a 10-km reach of the Missouri River at Sheepnose Bend, near Lexington, Missouri 2019-2021
The consists of longitudinal water surface profiles collected in a 10-km reach of the Missouri River at Sheepnose Bend downstream from Lexington, Missouri. Water surface elevation profiles were generated from data collected by USGS staff from the Columbia Environmental Research Center during multibeam sonar surveys of the channel bed in 2019, 2020, and 2021.
Soil chemistry adjacent to roads treated with dust control products at Loess Bluffs National Wildlife Refuge
The health of soils along roadways is critical for maximizing habitat quality and minimizing negative ecological effects of roads. Adjacent to unpaved roads, soil chemistry may be altered by the deposition of dust, as well as by road treatment with dust suppressants or soil stabilizer products. If present in roadside soils, these product residues may be available to plants, terrestrial invertebrates, or small mammals. Unfortunately, very few studies have attempted to track the transport of dust suppressants after application. As part of a larger ongoing study on the environmental effects of dust suppressant products on roadside plants and animals, we sampled roadside soils at Loess Bluff National Wildlife Refuge...
Black Carp in North America: a description of range, habitats, time of year, and methods of reported captures, May 2015-September 2018
Data represent reports of capture of black carp by commercial fishers and biologists with information regarding size characteristics of collected individuals, dimensions of capture gears, and spatial and temporal distributions of captures.
The vernal pool fairy shrimp, Branchinecta lynchi, (Branchiopoda; Anostraca) and other fairy shrimp species have been listed as ‘threatened’ or ‘endangered’ under the United States’ Endangered Species Act (ESA). There is little information about the sensitivity of Branchinecta spp. to toxic effects of contaminants, making it difficult to determine whether they are adequately protected by water quality criteria. A series of acute (24 hr) lethality/immobilization tests were conducted with three species of fairy shrimp (Branchinecta lynchi, Branchinecta lindahli, and Thamnocephalus platyurus) and 10 chemicals with varying modes of toxic action: ammonia, potassium, chloride, sulfate, chromium(VI), copper, nickel, zinc,...
Physical, Chemical, and Bioassay Data from the Study on Effects of Elevated Major Ions in Surface Water Contaminated by a Produced Water from Oil Production
This dataset characterizes sensitivity of fathead minnow and a unionid mussel to elevated major ions in produced water from oil and gas extraction process.
Survival and growth of four species of juvenile mussels in a pulsed flow-through auto-feeding beaker system.
Pebble count-based bed surface sediment grain size measurements from reach of the Big River near Washington State Park, Missouri
Pebble counts were performed to assess the bed surface grain size distribution at different habitats within a reach of the Big River near Washington State Park, Missouri. These data were collected in support of research assessing the hydraulic conditions of freshwater mussel habitat in the Big River. One component of this investigation was to examine the theoretical stability and instability of sediment particle sizes that are present within mussel habitats as well as within nearby reference habitats. These pebble counts furnished grain size data used for reference sediment stability predictions. Pebble counts were performed at seven different habitats in a study reach on the Big River following the methods of Wolman...
Response of juvenile mussels and amphipods to metal concentrations in water and sediment of streams draining the Tri-State Mining District, Missouri, Kansas and Oklahoma, USA- H. azteca and L. siliquoidea in long term exposures
This data release includes data from laboratory bioassay sediment exposures to Lampsilis siliquoidea and Hyallela azteca. Sediment was collected from streams and rivers within the Tri-State Mining District (TSMD). Endpoints include survival, reproduction and growth, metal concentrations, and various water quality parameters collected during the exposures
Chemical analyses and histopathology of organisms and plants collected from breccia pipe uranium mine sites in the Grand Canyon watershed, 2015-2020
These data are comprised of measurements of aluminum, iron, cobalt, nickel, copper, zinc, arsenic, selenium, molybdenum, silver, cadmium, thallium, lead, bismuth, thorium, uranium, and mercury in invertebrates, vegetation, and small rodents. Gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th) are also presented for vegetation; radioactivities for small rodents were previously presented at https://doi.org/10.5066/P9HARTQ4. Histopathology results are given for liver and kidney lesions generally associated with metals toxicoses in small rodents. Samples were collected at the Pinenut Mine (active mining) and Arizona 1 Mine (post-production) in the Grand Canyon watershed....