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Description of Work This spring (2014) we will measure Asian carp eDNA over time at a Missouri River site downstream of multiple spawning areas before and during spawning. We will measure the amount of Asian carp eDNA in water samples. The amount of eDNA will be related to the numbers of eggs and larvae counted in matched samples (water samples taken at the same time and place). The time since the eDNA was released by the carp will be estimated and these results will be related to the average age of AC eggs and larvae in matched samples. We will also test for substances which interfere with the eDNA measurement techniques and can lead to false negatives. We will also be validating markers developed by USGS and...
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Description of Work Initial tests of a variety of chemical stimuli identified a strong response to the algal food attractant. Field testing of chemical stimulants based on algae will seek to identify potent mixtures based on persistence and duration of attraction. These studies will include consideration of component chemicals such as amino acids produced by algae that enhance the attractiveness of the stimulus, based on carp smell and taste senses. Means of providing a sustained release of the stimulant will be explored through tests of various media. Tests will be conducted to confirm the possibility that carp can be conditioned to feeding stations that can be used to facilitate their capture. Relevance & Impact...
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Data represent reports of capture of black carp by commercial fishers and biologists with information regarding size characteristics of collected individuals, dimensions of capture gears, and spatial and temporal distributions of captures.
Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environment. Understanding the environmental factors that influence the decay of eDNA and how these factors impact detection probabilities over time and space could have significant implications for eDNA survey design and data interpretation. Here we experimentally explore eDNA decay in waste materials and reproductive cells obtained...
These data are comprised of measurements of gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th, Ra-226) in small rodent bodies. Samples were collected at non-mining and U breccia pipe mining sites in various production stages, in the Grand Canyon watershed.
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Data collected from a comprehensive set of hydrodynamic experiments conducted with live grass carp eggs and larvae, to evaluate drifting and swimming patterns with 3 different in-stream obstructions: (1) a gravel bump, (2) a single cylinder, and (3) submerged vegetation. The hydrodynamic behavior of eggs and larvae with each obstruction was continuously monitored for about 85 consecutive hours. Transient spatial distributions of the locations of eggs and larvae across the water column were generated for each flow scenario.
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
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These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.), major anions (chloride, nitrite+nitrate as nitrogen, sulfate, etc.), dissolved organic carbon, and general water quality characteristics in Canyon Mine containment pond water samples collected in calendar year 2019.
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This data set includes a point cloud, digital surface model (DSM), and orthoimagery of the Fighter Strips at Palmyra Atoll, USA. Products were generated from aerial images collected in October 2016 using Unoccupied Aerial Systems (UAS, or "drones") with a Ricoh GRII digital camera. Imagery was processed using structure-from-motion photogrammetry that generates a three dimensional point cloud (.las) by identifying pixels from multiple images representing the same object and calculating the x, y, and z coordinates of that object/pixel. Point cloud data were processed to create a 6.3 cm resolution DSM (.tif) of the uppermost reflective surface (i.e., data have not been filtered for vegetation classes or height). Finally,...
Within large-river ecosystems, floodplains serve a variety of important ecological functions. A recent survey of 80 managers of floodplain conservation lands along the Upper and Middle Mississippi and Lower Missouri Rivers in the central United States found that the most critical information needed to improve floodplain management centered on metrics for characterizing depth, extent, frequency, duration, and timing of inundation. These metrics can be delivered to managers efficiently through cloud-based interactive maps. To calculate these metrics, we interpolated an existing one-dimensional HEC-RAS hydraulic model for the Middle Mississippi River, which simulated water surface elevations at cross sections spaced...
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Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of longnose darter DNA. Samples were collected from sites on the St. Francis river, Missouri in 2018.
Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.
Text files containing data regarding attributes of lake trout egg thiamine concentrations from sites in lakes Huron and Michigan collected during 2019-20, sampling site locations and quality assurance quality control values for comparison to previous years (2017-18) lake trout egg thiamine concentrations.
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Diploid and triploid Grass Carp (Ctenopharyngodon Idella) tagged with acoustic transmitters were observed in Truman Reservoir, Missouri from February 2017 to March 2019 to determine habitat usage in winter. Habitat parameters were measured at used and available sites to model habitat preference. In addition, tagged fish were targeted during removal attempts to determine the efficacy of using tagged Judas Grass Carp in removing wild conspecifics. This dataset includes information on tagged Grass Carp and habitats they selected as well as capture events and characteristics of the wild fish caught during these attempts.
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The consists of longitudinal water surface profiles collected in a 10-km reach of the Missouri River at Sheepnose Bend downstream from Lexington, Missouri. Water surface elevation profiles were generated from data collected by USGS staff from the Columbia Environmental Research Center during multibeam sonar surveys of the channel bed in 2019, 2020, and 2021.
The health of soils along roadways is critical for maximizing habitat quality and minimizing negative ecological effects of roads. Adjacent to unpaved roads, soil chemistry may be altered by the deposition of dust, as well as by road treatment with dust suppressants or soil stabilizer products. If present in roadside soils, these product residues may be available to plants, terrestrial invertebrates, or small mammals. Unfortunately, very few studies have attempted to track the transport of dust suppressants after application. As part of a larger ongoing study on the environmental effects of dust suppressant products on roadside plants and animals, we sampled roadside soils at Loess Bluff National Wildlife Refuge...
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Description of Work USEPA Region 5 previously funded a study in 2011 evaluating the toxicity of nitrogen compounds to a select number of pond-dwelling amphibians. The objective of this new study will be to determine the toxicity of nitrogen compounds to stream-dwelling amphibians. Nitrogen contamination in aquatic systems results from the decomposition of organic matter, as well as through agricultural inputs, sewage effluents and industrial activities. Nitrogen in its various forms is of ecological concern because of its ubiquitous distribution and toxicity to aquatic life. Nitrogen compounds pose a hazard to aquatic larval stages of amphibians, affecting survival and development, and inducing deformities and behavioral...
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These datasets describe channel adjustments over a 60-year period to dams in the Missouri National Recreational River (MNRR), South Dakota and Nebraska. Datasets include a channel geometry analysis from cross-sections, banklines digitized and interpreted to predict future bank erosion sites from aerial photography, and models of floodplain inundation over a range of discharges. There are two MNRR segments, a 39-mile segment downstream from Fort Randall Dam, and a 59-mile segment downstream from Gavins Point Dam. Similar datasets were analyzed for each MNRR segment using similar methods.
The vernal pool fairy shrimp, Branchinecta lynchi, (Branchiopoda; Anostraca) and other fairy shrimp species have been listed as ‘threatened’ or ‘endangered’ under the United States’ Endangered Species Act (ESA). There is little information about the sensitivity of Branchinecta spp. to toxic effects of contaminants, making it difficult to determine whether they are adequately protected by water quality criteria. A series of acute (24 hr) lethality/immobilization tests were conducted with three species of fairy shrimp (Branchinecta lynchi, Branchinecta lindahli, and Thamnocephalus platyurus) and 10 chemicals with varying modes of toxic action: ammonia, potassium, chloride, sulfate, chromium(VI), copper, nickel, zinc,...


map background search result map search result map Toxicity in Stream-Dwelling Amphibians Adaptation and establishment of microchemical methods to analyze elemental patterns in calcified structures of sturgeon for determining origins and movements of freshwater sturgeon in the Mississippi and Missouri river basins Orthoimagery and elevation data derived from UAS imagery for Palmyra Atoll, USA 2016-Fighter Strips Black Carp in North America: a description of range, habitats, time of year, and methods of reported captures Data for the assessment of the containment pond at Canyon Mine, Arizona, 2019-Water Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017 No Levee (Altered) Inundation Metrics for the Middle Mississippi River Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018 Thiamine concentrations in lake trout eggs collected from the Great Lakes in 2019-20 Water quality, habitat, sampling methods and characteristics for grass carp in Truman Reservoir Missouri, 2017-2019 Channel geometry, banklines and floodplain inundation over a range of discharges in two segments of the Missouri National Recreational River, South Dakota and Nebraska, 1955-2018 Water surface elevation longitudinal profile data for a 10-km reach of the Missouri River at Sheepnose Bend, near Lexington, Missouri 2019-2021 Data for the assessment of the containment pond at Canyon Mine, Arizona, 2019-Water Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus Orthoimagery and elevation data derived from UAS imagery for Palmyra Atoll, USA 2016-Fighter Strips Water surface elevation longitudinal profile data for a 10-km reach of the Missouri River at Sheepnose Bend, near Lexington, Missouri 2019-2021 Water quality, habitat, sampling methods and characteristics for grass carp in Truman Reservoir Missouri, 2017-2019 Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017 Channel geometry, banklines and floodplain inundation over a range of discharges in two segments of the Missouri National Recreational River, South Dakota and Nebraska, 1955-2018 No Levee (Altered) Inundation Metrics for the Middle Mississippi River Thiamine concentrations in lake trout eggs collected from the Great Lakes in 2019-20 Adaptation and establishment of microchemical methods to analyze elemental patterns in calcified structures of sturgeon for determining origins and movements of freshwater sturgeon in the Mississippi and Missouri river basins Black Carp in North America: a description of range, habitats, time of year, and methods of reported captures Toxicity in Stream-Dwelling Amphibians