Filters: partyWithName: Northern Rocky Mountain Science Center (X) > partyWithName: Adam J Sepulveda (X) > partyWithName: Patrick R Hutchins (X)

Filters Date Range Past year (0) Past month (0) Past week (0) Past day (0) Custom range... To   Search Types Citation (1) Contacts U.S. Geological Survey Ecosystems U.S. Geological Survey - ScienceBase GS ScienceBase Categories Data (1) Tag Types Place (3) Theme (3) Harvest Set (3) USGS Scientific Topic Keyword (1) Tag Schemes Common geographic areas (2) USGS Thesaurus (3) ISO 19115 Topic Category (2) NA (1) None (1) View Results as: JSON ATOM CSV

Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples. Types: Citation; Tags: Aquatic Biology, California, Genetics, Idaho, Montana, All tags... Tetracapsuloides bryosalmonae, USGS Science Data Catalog (SDC), Wildlife Disease, Wyoming, environmental DNA, molecular biology, parasites, Fewer tags PCR results from dreissenid mussel round robin assay analyses, 2018-2019 Real-time PCR results of a round robin evaluation of 5 assays that target dreissenid mussel DNA. Water samples collected from waters with and without dreissenid mussels were analyzed using these five assays in four USGS laboratories. Samples from waters without dreissenid mussels were spiked with known amounts of dreissend DNA. Categories: Data; Tags: California, Jackson Lake, Lake Mead, Lake Michigan, Lower Columbia, All tags... New York, USGS Science Data Catalog (SDC), Upper Columbia, Upper Yellowstone, biota, environment, environmental DNA, invasive species, molecular biology, Fewer tags T. bryosalmonae detection in fish and water, DNA sequence, and simple sequence repeat data collected in the Inter-Mountain West from 2011 to 2019 This data release contains molecular diagnostic results and molecular detection of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue and environmental DNA samples collected from the inter-mountain West, USA. Tags: California, Idaho, Montana, USGS Science Data Catalog (SDC), Upper Snake, All tags... Upper Yellowstone, aquatic biology, biota, ecology, farming, genetics, parasites, Fewer tags