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Filters: partyWithName: Northern Rocky Mountain Science Center (X) > partyWithName: Adam J Sepulveda (X)

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We collected diets on Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) and brown trout (Salmo trutta) above (Yellowstone cutthroat trout allopatry) and below (Yellowstone cutthroat trout sympatry with brown trout) a natural barrier in Duck Creek, Montana. We used gastric lavage methods to collect diets, and samples were placed in storage containers and taken back to our lab and frozen until sorting. All samples were sorted by Rhithron, Inc. (Missoula, MT) with individuals identified down to the order.
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We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
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Positive and negative dreissenid mussel DNA quantitiative PCR results from environmental DNA water samples collected in Montana, Wisconsin and Minnesota to assess if environmental DNA can extend the seasonal window for dreissenid mussel early detection.
This data release contains molecular diagnostic results and molecular detection of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue and environmental DNA samples collected from the inter-mountain West, USA.
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Real-time PCR results of a round robin evaluation of 5 assays that target dreissenid mussel DNA. Water samples collected from waters with and without dreissenid mussels were analyzed using these five assays in four USGS laboratories. Samples from waters without dreissenid mussels were spiked with known amounts of dreissend DNA.
Field estimates of the abundance of rainbow trout in Washington and British Columbia were collected in concert with environmental DNA samples (eDNA) to evaluate if eDNA copy numbers correlated with abundance of trout. In addition, stream habitat data including channel units (pools, riffles), substrate, large woody debris, among others, were collected at sites.
The dataset includes measurements of stream habitat, fish abundance of westslope cutthroat trout and bull trout, and species-specific measures of environmental DNA (eDNA) from within the water. The data covers multiple streams in western Montana.
Field estimates of the abundance of two trout species (bull trout and westslope cutthroat trout) in Montana and rainbow trout in Washington and British Columbia were collected in concert with environmental DNA samples (eDNA) to evaluate if eDNA copy numbers correlated with abundance of trout. In addition, stream habitat data including channel units (pools, riffles), substrate, large woody debris, among others, were collected at sites.
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Quantitative PCR data from mesocosm experiments and field evaluations comparing coarse filter-large water volume environmental DNA samples vs. fine filter-small water volume environmental DNA samples for detection and quantification of rainbow trout (Oncorhynchus mykiss) and bull trout (Salvelinus confluentus) DNA. Mesocosm experiments took place at the US Fish & Wildlife Service's Creston Fish Hatchery in Montana, 2016. Field surveys took place in the Flathead River basin of Montana, 2016.
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We used 7 microsatellites to characterize the invasion genetics of 528 American bullfrogs sampled from the Yellowstone River floodplain (Montana). American bullfrog samples were collected from a 140 km section of the Yellowstone River near Billings, MT in 2013 - 2017.
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We tested the sensitivity of a portable, field-based environmental DNA (eDNA) platform relative to widely used lab-based eDNA approaches for detecting invasive northern pike (Esox lucius) in eight lakes on Alaska’s Kenai Peninsula. Raw data reported in this dataset report detect/non-detect data for technical replicates of water samples.
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Environmental DNA results for water samples collected at USGS streamgages in the Columbia and Colorado river basins. Water samples were collected by USGS hydrologic technicians May - September 2018 and analyzed for presence of kokanee and yellow perch DNA. In this dataset, we report results and applicable covariates associated with each streamgage location. This is version 1.1 of this data release. The Kokanee_Perch_eDNA data and metadata files have been revised to correct for improper attribution of 16 samples. For access to previous versions, please contact the Point of Contact identified on this page.
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We collected lengths and weights of Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) and brown trout (Salmo trutta) above (Yellowstone cutthroat trout allopatry) and below (Yellowstone cutthroat trout sympatry with brown trout) a natural barrier in Duck Creek, Montana.
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Environmental DNA detection results from samples collected using autonomous water sampling robots and manual approaches. Samples were collected in the Upper Yellowstone River (Montana) and Upper Snake River (Idaho/Wyoming) in 2018 and 2019. Samples were tested for the DNA of the following species: the waterborne protozoa Naegleria spp., the fish pathogen Tetracapsuloides bryosalmonae, Scomber japonicas (mackerel fish), kokanee salmon (Oncorhynchus nerka) and dreissenid mussel (Dreissena spp.).
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We collected lengths and weights and diets of Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) and brown trout (Salmo trutta) above (Yellowstone cutthroat trout allopatry) and below (Yellowstone cutthroat trout sympatry with brown trout) a natural barrier in Duck Creek, Montana.
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Environmental DNA results for water samples collected at USGS streamgages in the Columbia and Colorado river basins. Water samples were collected by USGS hydrologic technicians May - September 2018 and analyzed for presence of dreissenid mussel DNA. In this dataset, we report quantitative PCR results for each streamgage location.
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Environmental DNA quantitative PCR results for water samples collected in 2018 at USGS streamgages downstream of Columbia River Basin reservoirs that are considered to have moderate to high suitability for invasive dreissenid mussels. Water samples were tested for DNA of dreissenid mussels, kokanee salmon, and yellow perch. This is version 1.1 of this data release. The Kokanee_Perch_eDNA data and metadata files have been revised to correct for improper attribution of 16 samples. For access to previous versions, please contact the Point of Contact identified on this page. First Posted March 29, 2019 Revised July 19, 2019, ver 1.1


    map background search result map search result map Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples Yellowstone Cutthroat Trout and Brown Trout capture-recapture and diet data from Duck Creek, MT in summer and fall (2013-2016) Yellowstone Cutthroat Trout and Brown Trout diet data from Duck Creek, MT in summer and fall 2015 Yellowstone Cutthroat Trout and Brown Trout capture-recapture data from Duck Creek, MT in summer and fall 2013-2016 Environmental DNA detection data of Northern pike (Esox lucius) using a portable, field-based platform and a lab-based platform Environmental DNA results from dreissenid mussel early detection surveys in Montana, Minnesota, and Wisconsin 2017-2018 Environmental DNA surveillance data for USGS streamgage sampling in the Columbia River Basin, 2018 Environmental DNA results for large and small volume water samples in mesocosm experiments at Creston Fish Hatchery and field surveys in the Flathead River Basin, MT in 2016 Dreissenid mussel eDNA results for streamgage water samples in the Columbia and Colorado River basin Fish eDNA results for streamgage water samples in the Columbia and Colorado River basins PCR results from dreissenid mussel round robin assay analyses, 2018-2019 Environmental DNA robotic and manual sampling data, Yellowstone and Snake Rivers, 2017-2019 Microsatellite data for American bullfrogs in the Yellowstone River Montana, 2013-2017 T. bryosalmonae detection in fish and water, DNA sequence, and simple sequence repeat data collected in the Inter-Mountain West from 2011 to 2019 Environmental DNA data, fish abundance data, and stream habitat data from northwest Montana and northeast Washington and southern British Columbia, Canada Fish abundance data, environmental DNA (eDNA) data, and stream habitat data from streams in western Montana Fish abundance, environmental DNA, and stream habitat data from Washington, and southern British Columbia, Canada Yellowstone Cutthroat Trout and Brown Trout capture-recapture and diet data from Duck Creek, MT in summer and fall (2013-2016) Yellowstone Cutthroat Trout and Brown Trout diet data from Duck Creek, MT in summer and fall 2015 Yellowstone Cutthroat Trout and Brown Trout capture-recapture data from Duck Creek, MT in summer and fall 2013-2016 Environmental DNA detection data of Northern pike (Esox lucius) using a portable, field-based platform and a lab-based platform Fish abundance data, environmental DNA (eDNA) data, and stream habitat data from streams in western Montana Environmental DNA results for large and small volume water samples in mesocosm experiments at Creston Fish Hatchery and field surveys in the Flathead River Basin, MT in 2016 Microsatellite data for American bullfrogs in the Yellowstone River Montana, 2013-2017 Fish abundance, environmental DNA, and stream habitat data from Washington, and southern British Columbia, Canada Environmental DNA robotic and manual sampling data, Yellowstone and Snake Rivers, 2017-2019 Environmental DNA data, fish abundance data, and stream habitat data from northwest Montana and northeast Washington and southern British Columbia, Canada T. bryosalmonae detection in fish and water, DNA sequence, and simple sequence repeat data collected in the Inter-Mountain West from 2011 to 2019 Environmental DNA results from dreissenid mussel early detection surveys in Montana, Minnesota, and Wisconsin 2017-2018 Environmental DNA surveillance data for USGS streamgage sampling in the Columbia River Basin, 2018 Dreissenid mussel eDNA results for streamgage water samples in the Columbia and Colorado River basin Fish eDNA results for streamgage water samples in the Columbia and Colorado River basins Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples PCR results from dreissenid mussel round robin assay analyses, 2018-2019