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The data set contains the results of experimental challenge of captive zebra finches with an American crow isolate of West Nile virus (WNV). Data include infectivity, mortality, viremia, oral shedding of virus, and serology for anti- WNV antibodies. Australian and Timor zebra finches were used in this study and both are useful as a laboratory model of an avian species with moderate susceptibility to WNV.
Categories: Data;
Types: Citation;
Tags: National Wildlife Health Center,
USGS Science Data Catalog (SDC),
West Nile virus,
immunoassay,
qRT-PCR,
The data set contains results for nine serum biochemistries in molting Pacific black brant (Branta bernicla nigricans). These data were used to calculate reference intervals (sometimes referred to as normal values) for the nine serum biochemistries. All brant were after-hatch year. All samples were collected in 2006 and 2007 in the Teshekpuk Lake Special Area, Alaska.
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Alaska,
Arctic Coastal Plain,
Serum biochemistry,
Teshekpuk Lake Special Area,
USGS Science Data Catalog (SDC)
The data set contains paired-end, 100 nucleotide long RNA sequencing reads for each sample. Raw sequencing reads ranged from 18-30million reads per sample. Quality trimmed reads were mapped to the Zebra Finch reference genome with an average of 79.0-80.8% mapping rate, corresponding to 18,618 Ensembl gene IDs. Of these, 14,114 genes averaged at least 5 mapped reads across all samples and were utilized for differential expression (DE) analyses. DE analyzed two ways: as pairwise comparisons between treatments to identify specific genes with DEseq2 and as a time course grouping genes into expression paths with EBSeqHMM.
Categories: Data;
Types: Citation;
Tags: National Wildlife Health Center,
RNAseq,
Transcriptome,
USGS Science Data Catalog (SDC),
West Nile virus
Bats occupying hibernacula during summer months may play an important role in the epidemiology of white-nose syndrome (WNS). For example, bats exposed to viable Pseudogymnoascus destructans (Pd), the causative agent of WNS, in late summer could spread the fungus by transmitting it to other bats or to other locations suitable for establishment of new environmental reservoirs. To explore risks for transmission of Pd during the non-hibernal season, we screened bats using hibernacula between July 18 to August 22, 2012 and associated environmental samples for presence of Pd. Study sites within the eastern United States included six hibernacula known to harbor Pd and two hibernacula in which bats with WNS had not been...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Indiana,
Kentucky,
Ohio,
Tennessee,
USGS Science Data Catalog (SDC),
Chronic Wasting Disease (CWD) is a fatal, contagious, neuro-degenerative disease affecting multiple members of the Family Cervidae. First detected in 1967, the disease has, as of March 2021, been documented in free-ranging and/or captive cervid populations in 26 states, three Canadian provinces, the Republic of South Korea, Norway, Sweden, and Finland. The data provided here contains information on the known, available, documented distribution of CWD in the United States that is current as of the publication date. The USGS National Wildlife Health Center (NWHC) tracks changes to the known distribution of CWD in the form of a map (available on the NWHC website at https://www.usgs.gov/centers/nwhc/science/expanding-distribution-chronic-wasting-disease.
We reviewed pathological findings and to a lesser extent epidemiological data from 70 free-ranging columbiforms naturally infected with Pigeon paramyxovirus-1 (PPMV-1) from 25 different PPMV-1 mortality events in columbiforms in the USA. In a subset of 17 birds from 10 of the studied outbreaks, we carried out immunohistochemistry targeting PPMV-1 nucleoprotein to determine the tissue distribution of the virus.
Categories: Data;
Types: Citation;
Tags: Arizona,
Eurasian collared-dove,
Kansas,
Massachusetts,
Mississippi,
To investigate the role of migratory birds in the dissemination of West Nile virus (WNV), we measured the prevalence of infectious WNV and specific WNV neutralizing antibodies in birds, principally Passeriformes, during spring and fall migrations in the Atlantic and Mississippi flyways from 2001-2003. Blood samples were obtained from 13,403 birds, representing 133 species. Specific WNV neutralizing antibody was detected in 254 resident and migratory birds, representing 39 species, and was most commonly detected in northern cardinals ( Cardinalis cardinalis ) (9.8%, N = 762) and gray catbirds ( Dumetella carolinensis ) (3.2%, N = 3188). West Nile virus viremias were detected in 19 birds, including 8 gray catbirds,...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Atlantic migratory bird flyway,
Mississippi migratory bird flyway,
USGS Science Data Catalog (SDC),
United States,
birds,
During 2010 to 2013, waterbird mortality surveillance programs used a shared protocol for shoreline walking surveys performed June to November at three areas in northern Lake Michigan. In 2010 and 2012, 1244 total carcasses (0.8 dead bird/km walked) and 2399 total carcasses (1.2 dead birds/km walked), respectively, were detected. Fewer carcasses were detected in 2011 (353 total carcasses, 0.2 dead bird/km walked) and 2013 (451 total carcasses, 0.3 dead bird/km walked). During 3 years, peak detection of carcasses occurred in October and involved primarily migratory diving and fish-eating birds, including long-tailed ducks (Clangula hyemalis; 2010), common loons (Gavia immer; 2012), and red-breasted mergansers (Mergus...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Clostridium botulinum,
Great Lakes,
USGS Science Data Catalog (SDC),
avian botulism,
surveillance,
Monkeypox virus (MPXV) is a zoonotic disease endemic in Central and West Africa and is the most virulent orthopoxvirus affecting humans since the eradication of variola virus (VARV). In order to assess their reservoir potential, wild-caught rope squirrels were experimentally infected via intranasal and intradermal exposure with a recombinant MPXV strain from Central Africa engineered to express the luciferase gene. After infection, we monitored viral replication via in vivo bioluminescent imaging and viral shedding via culture and PCR. MPXV infection in African rope squirrels caused mortality and moderate to severe morbidity, with clinical signs including pox lesions in the skin, eyes, mouth and nose, dyspnea, and...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Congo (Democratic Republic of the),
Monkeypox virus,
USGS Science Data Catalog (SDC),
biophotonic imaging,
serology,
In 2010, a mass mortality of pufferfish in Hawaii was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that 1) a marine toxin was associated with a kill of a fish species that is, itself, toxic; 2) we provide a plausible mechanism to explain clinical signs of affected fish, and 3) this epizootic likely depleted puffer populations. Whilst our data are compelling, we did not synthesize the toxin de-novo, we were unable to categorically prove that the polar toxins caused mortality or that they were metabolites of an undefined...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Dereplication,
Hawaii,
USGS Science Data Catalog (SDC),
pathology,
pathology,
(i) Severe perkinsea data was based on the observation of characteristic gross and microscopic lesions and the morphological identification of Perkinsea organisms within the lesions. Necropsies and gross evaluation of carcasses were carried out under a dissecting microscope. Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (ii) Epidemiological information was compiled from each anuran sample, including the detection type (mortality event or health monitoring study), location (state and county), anuran species reported in the event, life-stage of species reported in the...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: USGS Science Data Catalog (SDC),
United States,
amphibians,
disease vectors,
health and disease,
These data were collected as part of a field trial to test the efficacy of a sylvatic plague vaccine. Treatment and control sites were selected randomly from the available sites at each location. Site pairs were a minimum of 20 acres, (with a few exceptions). Prairie dog trapping took place a minimum of two weeks post-baiting and trapping procedures were approved by the NWHC Animal Care and Use Committee as well as individual states as required.
Categories: Data;
Types: Citation;
Tags: Arizona,
Awapa Plateau,
Buffalo Gap National Grasslands,
Cedar City,
Charles M. Russell National Wildlife Refuge,
Temperature-dependent growth of Geomyces destructans, the fungus that causes bat white-nose syndrome
White-nose syndrome (WNS) is an emergent disease estimated to have killed over five million North American bats. Caused by the psychrophilic fungus Geomyces destructans, WNS specifically affects bats during hibernation. We describe temperature-dependent growth performance and morphology for six independent isolates of G. destructans from North America and Europe. Thermal performance curves for all isolates displayed an intermediate peak with rapid decline in performance above the peak. Optimal temperatures for growth were between 12.5 and 15.8 degrees C, and the upper critical temperature for growth was between 19.0 and 19.8 degrees C. Growth rates varied across isolates, irrespective of geographic origin, and above...
Categories: Data;
Types: Citation;
Tags: Bats,
Curve fitting,
Fungal diseases,
Fungal physiology,
Fungal structure,
The authors screened 1,397 feral horses (Equus caballus) on Sheldon National Wildlife Refuge, Nevada, United States, for IgM and IgG against flavivirus during 2004-2006, 2008, and 2009. Positive serum samples were tested for neutralizing antibodies to West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). One animal was positive for antibody against WNV in 2004, but all others tested in 2004-2006 were negative. In 2008 and 2009, the authors found evidence of increasing seropositive horses with age, whereas seroprevalence of WNV decreased from 19% in 2008 to 7.2% in 2009. No horses were positive for antibody against SLEV. Being unvaccinated, feral horses can be useful for WNV surveillance.
Categories: Data;
Types: Citation;
Tags: Sheldon National Wildlife Refuge, Nevada, USA,
USGS Science Data Catalog (SDC),
West Nile virus,
antibody,
disease vectors,
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