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Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environment. Understanding the environmental factors that influence the decay of eDNA and how these factors impact detection probabilities over time and space could have significant implications for eDNA survey design and data interpretation. Here we experimentally explore eDNA decay in waste materials and reproductive cells obtained...
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This dataset includes microsomal EROD data from an assay done with liver samples from several fish species that are found in Arizona at sites that are being assessed for PBDE contamination. The data was created in September and October 2016.
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Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of Spectaclecase DNA. Samples were collected from sites on the Big Piney River, Missouri from 2020 to 2022.
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We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring...
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This data consists of information on the specificity and sensitivity of 4 species specific qPCR assays for the detection of eDNA from 4 species of freshwater mussels, specifically Lampsilis cardium, Lampsilis siliquoidea, Pyganodon grandis, and Anodontoides ferussacianus. The data also contain the results of eDNA sampling in Niobrara River at Niobrara National Scenic River and Agate Fossil Beds National Monument for detection of these 4 species.
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Data describe a designed environmental DNA (eDNA) experiment for the estimation of eDNA degradation rates for the mucket (Actinonaias ligamentina) from water samples collected in the laboratory. Samples were collected from experiments performed from November 01, 2019 to November 15, 2019 at the USGS Columbia Environmental Research Center, Missouri
Data describe a designed environmental DNA (eDNA) survey for the detection of oyster mussel (Epioblasma capsaeformis) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of oyster mussel DNA. Samples were collected from sites on the Clinch River, Tennessee from 2019 to 2021
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Data describe a designed environmental DNA (eDNA) experiment for the estimation of eDNA degradation rates for the spectaclecase (Cumberlandia monodonta) from water samples collected in the laboratory. Parameters described include laboratory collected water samples that were tested to estimate concentration of spectaclecase DNA and associated quality assurance data. Samples were collected from experiments performed from May 17, 2021 to May 30, 2021 at the USGS Columbia Environmental Research Center, Missouri


    map background search result map search result map Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data Microsomal EROD data of fish liver sample assay from species collected in the Salt and Gila Rivers, Arizona Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021) Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri (2020 – 2022) Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay Laboratory estimates of eDNA degradation rates for Actinonaias ligamentina eDNA Development, testing and use of four species-specific qPCR assays for freshwater mussel eDNA detection in the Niobrara River Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay Laboratory estimates of eDNA degradation rates for Actinonaias ligamentina eDNA Development, testing and use of four species-specific qPCR assays for freshwater mussel eDNA detection in the Niobrara River Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri (2020 – 2022) Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021)