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Bacterial Data include results of incubations of lakewater bacteria with dissolved organic matter extracted from Colorado glacier or rock glacier outflows in 2015. Cells were counted pre- and post- incubations. The “Counts” tab is the number of cells counted in each view of the microscope using the acridine orange method. “C Calculations” tab is the calculation of carbon as bacterial biomass from cell counts. ”Change in cell counts” is the difference in the number of bacterial cells from the pre-incubation microbial culture and in each assay bottle after the biological oxygen demand incubation. The third tab, “Carbon use efficiency” is calculated as the amount of DOM from oxygen demand assays converted to microbial...
The effects of a dilute (ionic strength = 5 × 10−3 M) plume of treated sewage, with elevated levels (3.9 mg/L) of dissolved organic carbon (DOC), upon the pH-dependency and magnitude of bacterial transport through an iron-laden, quartz sand aquifer (Cape Cod, MA) were evaluated using sets of replicate, static minicolumns. Compared with uncontaminated groundwater, the plume chemistry diminished bacterial attachment under mildly acidic (pH 5.0–6.5) in-situ conditions, in spite of the 5-fold increase in ionic strength and substantively enhanced attachment under more alkaline conditions. The effects of the hydrophobic neutral and total fractions of the plume DOC; modest concentrations of fulvic and humic acids (1.5...
This dataset contains data tables of laboratory quality-control data associated with environmental samples analyzed for microbiological constituents at the Ohio Water Microbiology Laboratory of the U.S. Geological Survey (USGS). The environmental samples were collected across the United States by USGS National Projects and projects in Water Science Centers. These quality-control data can be used to assess the quality of microbiological data for the associated environmental samples.
To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted...
The dataset includes Escherichia coli concentrations in water and sediment collected by James Fluke in the Middle Rio Grande in Albuquerque, NM, from 2017 to 2018.
Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene...
Chemical and microbiological results, quality assurance and quality control, site location, and method information for surface water, bed sediment, and wastewater effluent collected from 34 stream locations across Iowa (United States). Environmental samples were analyzed for a suite of 29 antibiotics, plated on selective media for 15 types of bacteria growth, and DNA was extracted from culture growth and used in downstream polymerase chain reaction (PCR) assays for the detection of 24 antibiotic resistance genes. Environmental field data were collected in-situ for water temperature (°C), specific conductivity (µs/cm), dissolved oxygen (mg/L), pH (standard units), and turbidity (FNU) using a multiparameter sonde...
Categories: Data;
Tags: Environmental Health,
Iowa,
USGS Science Data Catalog (SDC),
United States,
Water Quality,
This data release contains the inputs and outputs from the development of Escherichia coli (E. coli) concentration models for the four Missouri River sites near Omaha, Nebraska. Model development, models, and results are published in the associated U.S. Geological Survey (USGS) report Densmore and others, 2020. Load Estimator models (LOADEST) (Runkel and others, 2004) were developed from the first 5 years of data (July 2012–September 2016). Sites include Missouri River at NP Dodge Park at Omaha, Nebr. (USGS Site ID 412126095565201), Missouri River at Freedom Park Omaha, Nebr. (USGS Site ID 411636095535104), Missouri River near Council Bluffs, Iowa (USGS Site ID 06610505), and Missouri River near La Platte, Nebr....
This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Trenton, New Jersey. Microbial acetylene uptake in groundwater samples was established by mixing the groundwater with a defined mineral medium to supply nutrients and providing acetylene as the sole electron donor and carbon source. The structure of the microbial community in those enrichments was characterized as shown by...
Categories: Data;
Tags: Environmental Health,
Trenton West,
USGS Science Data Catalog (SDC),
Water Resources,
aquatic biology,
Bacteria de América del Sur, América Central y el Caribe .
The mobility and bioavailability of Se depend on its redox state, and reduction of Se oxyanions to less mobile, reduced species controls transport of this potentially toxic element in the environment. Stable isotope fractionation of Se is currently being developed as an indicator of Se immobilization through reduction. In this study, Se isotope fractionation resulting from reduction of Se(VI) and Se(IV) oxyanions by natural microbial consortia was measured in sediment slurry experiments under nearly natural conditions, with no substrate added. Experiments were conducted with a wide range of initial Se concentrations and with sediment and water from three locations with contrasting environmental settings. The products...
Categories: Publication;
Types: Citation;
Tags: Bacteria,
Selenium,
Stable isotopes,
Water contaminat
Oil and gas (OG) wastewaters are commonly disposed of by underground injection and previous research showed that activities at a disposal facility in West Virginia affected stream biogeochemistry and sediment microbial communities downstream from the facility. Microorganisms can control the fate and transport of organic and inorganic components of OG wastewater highlighting the need to characterize the effects of OG wastewater components on microbial activity. We conducted a series of aerobic microcosm experiments to assess the influence of high total dissolved solids (TDS) and hydraulic fracturing fluid additives (2,2-dibromo-3-nitrilopropionamide (DBNPA), a biocide, and ethylene glycol, an anti-scaling additive),...
Categories: Data;
Tags: Biochemistry,
DNA sequencing,
Energy Resources,
Environmental Health,
Fayette County,
This product consists of multiple tabular datasets and associated metadata for clinical status, gene transcripts, and lab results related to presence of Mycoplasma spp. in desert reptiles. To better understand immune responses to pathogenic infections, we conducted an experiment to quantify innate and induced immune responses using gene transcript profiles and measured induced antibody levels for Mycoplasma spp. in Mojave Desert tortoises (Gopherus agassizii). Data consists of: - Clinical Status Data - Clinical status of presence or absence of nasal discharge, eroded nares, or labored respiration in 15 captive tortoises classified as naive, exposed, or infected. - Gene Transcript Data - Gene transcription values...
The dataset includes Escherichia coli concentrations collected by the Bosque Ecosystem Monitoring Program in the Middle Rio Grande in Albuquerque, NM, from 2017 to 2020.
This study evaluated the use of medicated feeds to treat furunculosis infections caused by the bacterium Aeromonas salmonicida in lake herring (Coregonus artedi, Lesueur, 1818). The objectives of this two-phase study were to (1) determine if Coregonus artedi could be adequately and consistently infected with A. salmonicida by the methods of bath immersion or intraperitoneal (IP) injection, and (2) determine if the medicated feeds Oxytetracycline Dihydrate (Terramycin® 200 For Fish) and Florfenicol (Aquaflor®) could be used for the control of furunculosis caused by A. salmonicida. These datasets include water quality, mortality, feed weights and behavior, and qPCR data collected during both phases of the study.
The dataset contains Escherichia coli (E. coli) concentrations and loads that were measured and calculated from samples collected from the Little Blue River and its tributaries in Independence, MO. The data was collected during the 2010-2016 recreation seasons. The U.S. Geological Survey, in coordination with the City of Independence, collected the data for use in regression models to evaluation E. coli loads in the Little Blue River and its tributaries within the boundaries of the City of Independence. Further information on the methods and the results of the data collection and regression models can be found in the conference proceedings paper cited below. Citation: Flickinger, A.; Christensen, E. Evaluation...
Categories: Data;
Types: Citation;
Tags: East Fork Little Blue River,
Independence, Missouri,
Little Blue River,
bacteria,
water-quality data
Description of Work The invasive form of Phragmites australis (common reed) is a well-established pest in many parts of the Great Lakes and the Atlantic, Gulf, and Pacific coasts, including designated Areas of Concern. New innovative control options that sustainably target the competitive advantage often enjoyed by Phragmites and other invasive plants will contribute to a broad Integrated Pest Management (IPM) strategy. This project targets the microorganisms that may help Phragmites spread and will employ a molecular genetic approach to silence the genes in Phragmites that give it a competitive edge over many native plants. This project helped build and will continue to be closely aligned with the Great Lakes Phragmites...
Categories: Project;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Areas of concern,
Ecosystem health,
GLRI,
Governmental,
Great Lakes Restoration Initiative,
Soil organic matter (SOM) biomarker methods were utilized in this study to investigate the responses of fungi and bacteria to freeze?thaw cycles (FTCs) and to examine freeze?thaw-induced changes in SOM composition and substrate availability. Unamended, grass-amended, and lignin-amended soil samples were subject to 10 laboratory FTCs. Three SOM fractions (free lipids, bound lipids, and lignin-derived phenols) with distinct composition, stability and source were examined with chemolysis and biomarker Gas Chromatography/Mass Spectrometry methods and the soil microbial community composition was monitored by phospholipid fatty acid (PLFA) analysis. Soil microbial respiration was also measured before and during freezing...
Categories: Publication;
Types: Citation,
Journal Citation;
Tags: Soil Biology and Biochemistry,
bacteria,
biomarkers,
bound lipids,
co2 flush,
Water-quality data were collected by the Providence Water Supply Board (PWSB) from tributaries in the Scituate Reservoir drainage area, October 2016 through September 2017 (water year 2017). Water-quality samples were collected by the PWSB either monthly or quarterly at fixed stations on 36 tributaries in the Scituate Reservoir drainage area. These data were used to calculate instantaneous loads and yields of constituents in a report by the U.S. Geological Survey. Water-quality samples were collected following a strict sampling schedule so that water-quality samples would be representative of various weather conditions. Samples were analyzed at the PWSB water-quality laboratory at the P.J. Holton Water Purification...
Bacteria de América del Sur, América Central y el Caribe .
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