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We collected diets on Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) and brown trout (Salmo trutta) above (Yellowstone cutthroat trout allopatry) and below (Yellowstone cutthroat trout sympatry with brown trout) a natural barrier in Duck Creek, Montana. We used gastric lavage methods to collect diets, and samples were placed in storage containers and taken back to our lab and frozen until sorting. All samples were sorted by Rhithron, Inc. (Missoula, MT) with individuals identified down to the order.
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We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
This dataset contains simple sequence repeat genotypes from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA.
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Positive and negative dreissenid mussel DNA quantitiative PCR results from environmental DNA water samples collected in Montana, Wisconsin and Minnesota to assess if environmental DNA can extend the seasonal window for dreissenid mussel early detection.
This dataset contains molecular diagnostic results of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue collected from the inter-mountain West, USA.
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Real-time PCR results of a round robin evaluation of 5 assays that target dreissenid mussel DNA. Water samples collected from waters with and without dreissenid mussels were analyzed using these five assays in four USGS laboratories. Samples from waters without dreissenid mussels were spiked with known amounts of dreissend DNA.
This data release contains molecular diagnostic results and molecular detection of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue and environmental DNA samples collected from the inter-mountain West, USA.
Field estimates of the abundance of rainbow trout in Washington and British Columbia were collected in concert with environmental DNA samples (eDNA) to evaluate if eDNA copy numbers correlated with abundance of trout. In addition, stream habitat data including channel units (pools, riffles), substrate, large woody debris, among others, were collected at sites.
Climate change may facilitate the expansion of non-native invasive species (NIS) in aquatic and terrestrial systems. However, empirical evidence remains scarce and poorly synthesized at scales necessary for effective management. We conducted a literature synthesis to assess the state of research on the observed and predicted effects of climate change on a suite of 398 aquatic and terrestrial NIS now present in or a major threat to aquatic and terrestrial ecosystems of the Pacific Northwest (PNW), USA and British Columbia. Surprisingly, very few studies (n = 15) have investigated the observed effects of climate change on the distribution, abundance, spread, or impact of the focal NIS, with only five studies focusing...
Field estimates of the abundance of two trout species (bull trout and westslope cutthroat trout) in Montana and rainbow trout in Washington and British Columbia were collected in concert with environmental DNA samples (eDNA) to evaluate if eDNA copy numbers correlated with abundance of trout. In addition, stream habitat data including channel units (pools, riffles), substrate, large woody debris, among others, were collected at sites.
The dataset includes measurements of stream habitat, fish abundance of westslope cutthroat trout and bull trout, and species-specific measures of environmental DNA (eDNA) from within the water. The data covers multiple streams in western Montana.
Invasive alien species (IAS) are a rising threat to biodiversity, national security, and regional economies, with impacts in the hundreds of billions of U.S. dollars annually. Proactive or predictive approaches guided by scientific knowledge are essential to keeping pace with growing impacts of invasions under climate change. Although the rapid development of diverse technologies and approaches has produced tools with the potential to greatly accelerate invasion research and management, innovation has far outpaced implementation and coordination. Technological and methodological syntheses are urgently needed to close the growing implementation gap and facilitate interdisciplinary collaboration and synergy among...
Categories: Publication; Types: Citation
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The headwaters of the Columbia River Basin in the Northern Rocky Mountains region is widely recognized as a stronghold for native fish, containing some of the last remaining connected cold-water habitats for species such as the threatened bull trout and native westslope cutthroat trout. However, as temperatures rise, non-native invasive fish species could be poised to prosper in the region as conditions start to favor warm-water species over those that require cooler waters to thrive. The spread of invasive fish species has the potential to devastate native fish populations, stream habitats, and the local cultures and economies that depend on healthy aquatic ecosystems – including the region’s multi-billion dollar...
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U.S. Geological Survey decision analysts and technical experts worked with representatives from the U.S. Bureau of Reclamation, Utah Division of Wildlife Resources, and Central Utah Water Conservancy District to predict the consequences of alternative actions following environmental DNA detections of dreissenid mussel eDNA in Jordanelle Reservor, Utah. This dataset provides the consequence and model inputs for each of the alternative actions under different levels of uncertainty.
This dataset contains DNA sequences of two genomic regions, cytochrome oxidase subunit one and internally transcribed spacer region one, obtained from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA.
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Quantitative PCR data from mesocosm experiments and field evaluations comparing coarse filter-large water volume environmental DNA samples vs. fine filter-small water volume environmental DNA samples for detection and quantification of rainbow trout (Oncorhynchus mykiss) and bull trout (Salvelinus confluentus) DNA. Mesocosm experiments took place at the US Fish & Wildlife Service's Creston Fish Hatchery in Montana, 2016. Field surveys took place in the Flathead River basin of Montana, 2016.
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We used 7 microsatellites to characterize the invasion genetics of 528 American bullfrogs sampled from the Yellowstone River floodplain (Montana). American bullfrog samples were collected from a 140 km section of the Yellowstone River near Billings, MT in 2013 - 2017.
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We tested the sensitivity of a portable, field-based environmental DNA (eDNA) platform relative to widely used lab-based eDNA approaches for detecting invasive northern pike (Esox lucius) in eight lakes on Alaska’s Kenai Peninsula. Raw data reported in this dataset report detect/non-detect data for technical replicates of water samples.
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Field data of age-0 smallmouth bass (Micropterus dolomieu) estimated age, lengths and weights, distribution from field sampling, and results from environmental DNA surveys from the Yellowstone River in Montana. Data were used in conjunction with existing stream temperature data from USGS gages to evaluate if temperature is related to age-0 size at the onset of winter.


map background search result map search result map Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples Yellowstone Cutthroat Trout and Brown Trout diet data from Duck Creek, MT in summer and fall 2015 Environmental DNA detection data of Northern pike (Esox lucius) using a portable, field-based platform and a lab-based platform Predicting Climate-Induced Expansions of Invasive Fish in the Pacific Northwest: Implications for Climate Adaptation of Native Salmon and Trout Environmental DNA results from dreissenid mussel early detection surveys in Montana, Minnesota, and Wisconsin 2017-2018 Environmental DNA results for large and small volume water samples in mesocosm experiments at Creston Fish Hatchery and field surveys in the Flathead River Basin, MT in 2016 PCR results from dreissenid mussel round robin assay analyses, 2018-2019 Microsatellite data for American bullfrogs in the Yellowstone River Montana, 2013-2017 T. bryosalmonae detection in fish and water, DNA sequence, and simple sequence repeat data collected in the Inter-Mountain West from 2011 to 2019 Environmental DNA data, fish abundance data, and stream habitat data from northwest Montana and northeast Washington and southern British Columbia, Canada Cytochrome oxidase subunit I (COI) and internally transcribed spacer region I (ITS1) DNA sequences of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019 Molecular detection of Tetracapsuloides bryosalmonae DNA from fish tissues and environmental DNA water samples collected throughout the Inter-mountain West, USA from 2011 to 2019 Simple sequence repeats (SSRs) of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019 Fish abundance data, environmental DNA (eDNA) data, and stream habitat data from streams in western Montana Fish abundance, environmental DNA, and stream habitat data from Washington, and southern British Columbia, Canada Juvenile Smallmouth Bass Sampling from the Yellowstone River, Montana 2016-2019 Predicted consequences of detecting dreissenid mussel eDNA in Jordanelle Reservoir Utah, 2021 Environmental DNA water sample analyses for smallmouth bass surveillance, Gardner River, Montana, March 2022 Yellowstone Cutthroat Trout and Brown Trout diet data from Duck Creek, MT in summer and fall 2015 Environmental DNA water sample analyses for smallmouth bass surveillance, Gardner River, Montana, March 2022 Predicted consequences of detecting dreissenid mussel eDNA in Jordanelle Reservoir Utah, 2021 Environmental DNA detection data of Northern pike (Esox lucius) using a portable, field-based platform and a lab-based platform Fish abundance data, environmental DNA (eDNA) data, and stream habitat data from streams in western Montana Environmental DNA results for large and small volume water samples in mesocosm experiments at Creston Fish Hatchery and field surveys in the Flathead River Basin, MT in 2016 Microsatellite data for American bullfrogs in the Yellowstone River Montana, 2013-2017 Fish abundance, environmental DNA, and stream habitat data from Washington, and southern British Columbia, Canada Juvenile Smallmouth Bass Sampling from the Yellowstone River, Montana 2016-2019 Environmental DNA data, fish abundance data, and stream habitat data from northwest Montana and northeast Washington and southern British Columbia, Canada Cytochrome oxidase subunit I (COI) and internally transcribed spacer region I (ITS1) DNA sequences of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019 Simple sequence repeats (SSRs) of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019 T. bryosalmonae detection in fish and water, DNA sequence, and simple sequence repeat data collected in the Inter-Mountain West from 2011 to 2019 Molecular detection of Tetracapsuloides bryosalmonae DNA from fish tissues and environmental DNA water samples collected throughout the Inter-mountain West, USA from 2011 to 2019 Environmental DNA results from dreissenid mussel early detection surveys in Montana, Minnesota, and Wisconsin 2017-2018 Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples Predicting Climate-Induced Expansions of Invasive Fish in the Pacific Northwest: Implications for Climate Adaptation of Native Salmon and Trout PCR results from dreissenid mussel round robin assay analyses, 2018-2019