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  4. Quantification of nitrogen-fixing microorganisms (targeting nifH gene) in Cladophora algae by quantitative polymerase chain reaction (qPCR)

Quantification of nitrogen-fixing microorganisms (targeting nifH gene) in Cladophora algae by quantitative polymerase chain reaction (qPCR)

Dates

Publication Date
Start Date
2015-06-18
End Date
2015-09-23

Citation

Byappanahalli, M.N., Przybyla‐Kelly, K., Ishii, S., and Aunins, A.W., 2019, Quantification of nitrogen‐fixing microorganisms (targeting the nifH gene) in Cladophora algae by quantitative polymerase chain reaction (qPCR): U.S. Geological Survey data release, https://doi.org/10.5066/P9U2SYRI.

Summary

Data were collected to determine the abundance of nitrogen-fixing microorganisms in Cladophora algae growing on rocks, breakwall structures, or submerged dreissenid mussel beds around southern Lake Michigan. Cladophora samples (N=33) were collected between June and September 2015 from three urban areas: (a) Jeorse Park, East Chicago, Indiana, (b) Calumet Beach, Chicago, Illinois, and (c) North Beach, Racine, Wisconsin, and a National Park site, Portage Lakefront, Indiana Dunes National Park, Indiana. Corresponding lake water samples (N=33) were collected approximately 15-20 feet away from submerged algal mats. Genomic DNA was extracted from water and processed algal samples. The abundance of nitrogen-fixing microorganisms in water [...]

Contacts

Point of Contact :
Muruleedhara Byappanahalli
Process Contact :
Katarzyna Przybyla-Kelly, Dawn A Shively, Satoshi Ishii
Originator :
Muruleedhara Byappanahalli, Katarzyna Przybyla-Kelly, Satoshi Ishii, Aaron W Aunins
Metadata Contact :
Muruleedhara Byappanahalli
Publisher :
U.S. Geological Survey
Distributor :
GS ScienceBase, U.S. Geological Survey
USGS Mission Area :
Ecosystems
SDC Data Owner :
Great Lakes Science Center

Attached Files

Click on title to download individual files attached to this item.

NifH_v5-1.xml
Original FGDC Metadata
View 		31.2 KB application/fgdc+xml
Lat_Long.csv 516 Bytes text/csv
PowerBiofilm.pdf 699.02 KB application/pdf
PowerWater.pdf 59.61 KB application/pdf
NifH_qPCR.csv 4.65 KB text/csv
NifH_taxonomicID.csv 40.78 KB text/csv

Purpose

The data were collected to determine the abundance of nitrogen-fixing microorganisms by targeting the nifH gene in actively growing (i.e., fresh) Cladophora algae in Lake Michigan. There is a significant knowledge gap in the abundance, taxonomic diversity, and nitrogen-fixing capabilities of these microorganisms. This information is critical for two reasons: first, to explain Cladophora’s nitrogen needs; second, to develop strategies to manage the nuisance algal problem by reducing nutrients entering the lakes via streams and other associated waterways.

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  • Great Lakes Science Center (GLSC)
  • USGS Data Release Products

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9U2SYRI

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