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Quantitative PCR detection of Batrachochytrium dendrobatidis DNA from sediments and water

Dates

Year
2007

Citation

Kirshtein, Julie D, Anderson, Chauncey W, Wood, John S, Longcore, Joyce E, and Voytek, Mary A, 2007, Quantitative PCR detection of Batrachochytrium dendrobatidis DNA from sediments and water: Diseases of Aquatic Organisms, v. 77, iss. 1, p. 11-15.

Summary

ABSTRACT: The fungal pathogen Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a disease implicated in amphibian declines on 5 continents. Polymerase chain reaction (PCR) primer sets exist with which amphibians can be tested for this disease, and advances in sampling techniques allow non-invasive testing of animals. We developed filtering and PCR based quantitative methods by modifying existing PCR assays to detect Bd DNA in water and sediments, without the need for testing amphibians; we tested the methods at 4 field sites. The SYBR based assay using Boyle primers (SYBR/Boyle assay) and the Taqman based assay using Wood primers performed similarly with samples generated in the laboratory (Bd spiked filters), but the SYBR/Boyle [...]

Contacts

Communities

  • USGS National Research Program

Tags

Provenance

Added to ScienceBase on Thu Apr 18 13:36:54 MDT 2013 by processing file <b>Hydrogeochemical and Microbiological Investigations of Radionuclides, Nutrients, and Environmental Contaminants.xml</b> in item <a href="https://www.sciencebase.gov/catalog/item/5042163ae4b04b508bfd3332">https://www.sciencebase.gov/catalog/item/5042163ae4b04b508bfd3332</a>

Additional Information

Identifiers

Type Scheme Key
DOI http://sciencebase.gov/vocab/identifierScheme 10.3354/dao01831

Citation Extension

citationTypeJournal Article
journalDiseases of Aquatic Organisms
parts
typePages
value11-15
typeVolume
value77
typeIssue
value1

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