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David M. Reinitz

Batrachochytrium dendrobatidis is a fungal pathogen that is receiving attention around the world for its role in amphibian declines. Study of its occurrence patterns is hampered by false negatives: the failure to detect the pathogen when it is present. Occupancy models are a useful but currently underutilized tool for analyzing detection data when the probability of detecting a species is <1. We use occupancy models to evaluate hypotheses concerning the occurrence and prevalence of B. dendrobatidis and discuss how this application differs from a conventional occupancy approach. We found that the probability of detecting the pathogen, conditional on presence of the pathogen in the anuran population, was related to...
Categories: Publication; Types: Citation; Tags: Ecological Applications
Increased reporting of amphibian malformations in North America has been noted with concern in light of reports that amphibian numbers and species are declining worldwide. Ribeiroia ondatrae has been shown to cause a variety of types of malformations in amphibians. However, little is known about the prevalence of R. ondatrae in North America. To aid in conducting field studies of Ribeiroia spp., we have developed a polymerase chain reaction (PCR)-based diagnostic. Herein, we describe the development of an accurate, rapid, simple, and cost-effective diagnostic for detection of Ribeiroia spp. infection in snails (Planorbella trivolvis). Candidate oligonucleotide primers for PCR were designed via DNA sequence analyses...
Categories: Publication; Types: Citation; Tags: Journal of Parasitology
We found amphibian chytrid fungus (Bd = Batrachochytrium dendrobatidis) to be widespread within a coastalwatershed at Point Reyes National Seashore, California and within two high elevation watersheds at Yosemite NationalPark, California. Bd was associated with all six species that we sampled (Bufo boreas, B. canorus, Pseudacris regilla, Ranadraytonii, R. sierrae, and Lithobates catesbeianus). For those species sampled at 10 or more sites within a watershed, thepercentage of Bd-positive sites varied from a low of 20.7% for P. regilla at one Yosemite watershed to a high of 79.6% forP. regilla at the Olema watershed at Point Reyes. At Olema, the percent of Bd-positive water bodies declined each year ofour study (2005-2007)....
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