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Joel G Putnam

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Description of Work This work began by assembling fish toxicant data into a common database and examining the database for trends in toxicant data. The trends found in the toxicant database were linked to physiochemical properties of the individual toxicants. Combining the toxicant trends with the physiochemical properties may allow for the prediction of toxicity and the identification of chemicals with selectivity for the Asian carp. Identifying a selective toxicant may provide control of Asian carps while minimizing effects on other non-target aquatic species. When Asian carp specific toxicants are identified from the initial screen within the database these toxicants are then further screened in toxicity assays...
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Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of population declines are often enigmatic with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event in the Clinch River, USA. Histopathology and transmission electron microscopy showed a novel microsporidian parasite primarily infecting the ovary of pheasantshell. Sequencing of the small subunit rRNA gene produced a 1333 bp sequence with greatest similarity to Pseudonosema cristatellae (AF484694.1; 86.36%; e-value = 0), a microsporidium...
This was a joint project between: Upper Midwest Environmental Sciences Center, U.S. Geological Survey, La Crosse, WI 54603 La Crosse Fish Health Center, U.S. Fish and Wildlife Service, Onalaska, WI 54650 This repository contains the raw data and R codes used in the metabolomics analysis for the manuscript which used silver and bighead carp fry cells exposed to thiram. Species indclude in this manuscript Silver Carp (Hypophthalmichthys molitrix) Bighead Carp (Hypophthalmichthys nobilis) The raw data is in: BHC thiram data.csv SVC thiram data labels.csv The code used in the ordination analysis is in: figure_1_knit_code.Rmd The code used in the principle component analysis is in: figure_2_knit_code.Rmd The...
Categories: Data
This data is toxicity data for fish exposed to thiram. The number of trials and species used are: 8 trials on Bighead carp (Hypophthalmichthys nobilis), 8 trials on Bluegill Sunfish (Lepomis macrochirus), 4 trials on Fathead minnow (Pimephales promelas), 5 trials on Rainbow Trout (Oncorhynchus mykiss), and 7 trials on Silver Carp (Hypophthalmichthys molitrix). The drc package from the R software was used to create an on-line web application. The combination of the web application and the toxicity data collected was used to suggest thiram, a fungicide, would be a selective toxiciant for bighead carp.
We combine cell assays and metabolomics to create a powerful tool, which emerges to elevate the identification of new control chemicals. We combined the use of bigheaded carp fry cell line with metabolite profiling to describe the dose response to thiram. Thiram is a registered pesticide commonly used as a fungicide in the field or as a seed protectant and is known to be toxic to fish. Seven concentrations of thiram were used to dose bighead carp fry cells and silver carp fry cells. We identified 700 metabolomic markers and 41 of those markers exhibited a dose response to thiram in the bighead carp fry cells. We identified 1590 metabolomic markers with 205 of those markers exhibited a dose response to thiram in...
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