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Person

Adam J Sepulveda

Biologist
Northern Rocky Mountain Science Center

Email: asepulveda@usgs.gov
Office Phone: 406-994-7975
Fax: 406-994-6556
ORCID: 0000-0001-7621-7028

Location
The Transportation and Systems
2327 University Way
Suite 2
Bozeman , MT 59715
US

Supervisor: Clint C Muhlfeld
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We collected diets on Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) and brown trout (Salmo trutta) above (Yellowstone cutthroat trout allopatry) and below (Yellowstone cutthroat trout sympatry with brown trout) a natural barrier in Duck Creek, Montana. We used gastric lavage methods to collect diets, and samples were placed in storage containers and taken back to our lab and frozen until sorting. All samples were sorted by Rhithron, Inc. (Missoula, MT) with individuals identified down to the order.
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Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
This dataset contains simple sequence repeat genotypes from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA.
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Positive and negative dreissenid mussel DNA quantitiative PCR results from environmental DNA water samples collected in Montana, Wisconsin and Minnesota to assess if environmental DNA can extend the seasonal window for dreissenid mussel early detection.
This dataset contains molecular diagnostic results of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue collected from the inter-mountain West, USA.
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