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Person

Rebecca A Cole

Zoologist

National Wildlife Health Center

Email: rcole@usgs.gov
Office Phone: 608-270-2468
Fax: 608-270-2415
ORCID: 0000-0003-2923-1622

Location
6006 Schroeder Road
Madison , WI 53711-6223
US

Supervisor: David S Blehert
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Animal identification number, weight in grams for carcasses of California tiger salamanders (Ambystoma californiense) CTS and Santa Cruz long-toed salamander (Ambystoma macrodactylum croceum) SCLT from Prospect or Ellicott Pond, on Ellicott Slough National Wildlife Refuge, California U.S.A. submitted to U.S. Geological Survey, National Wildlife Health Center (NWHC) for determination of cause of death. NWHC Case 29381. MSF Sequence file used to construct a phylogenetic tree. Sequences were generated from samples at NWHC (Px) and using BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) they were compared for base pair similarities with publicly available sequences from GenBank. Sequences selected for the Multi Sequence...
Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls.
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Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of population declines are often enigmatic with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event in the Clinch River, USA. Histopathology and transmission electron microscopy showed a novel microsporidian parasite primarily infecting the ovary of pheasantshell. Sequencing of the small subunit rRNA gene produced a 1333 bp sequence with greatest similarity to Pseudonosema cristatellae (AF484694.1; 86.36%; e-value = 0), a microsporidium...
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